Reactivity | AllSpecies Glossary |
Applications | WB, Flow, ICC/IF, IHC, IHC-P |
Clone | Hyb-8 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | DyLight 755 |
Immunogen | Biotinylated sheep immunoglobulin |
Localization | Depends on the target |
Specificity | It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Store at 4C in the dark. |
Buffer | 50mM Sodium Borate |
Preservative | 0.05% Sodium Azide |
Purity | Protein A or G purified |
Publication using NBP2-34646IR | Applications | Species |
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Kumar S, Park H, Cho H et al. Overcoming evanescent field decay using 3D-tapered nanocavities for on-chip targeted molecular analysis Nat Commun Jun 10 2020 [PMID: 32523000] (ICC/IF, Bovine) | ICC/IF | Bovine |
Secondary Antibodies |
Isotype Controls |
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