Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) [DyLight 755]

• Reactivity: All
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: Hyb-8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 755

Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) [DyLight 755] Summary

• Immunogen: Biotinylated sheep immunoglobulin
• Localization: Depends on the target
• Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Use in Bovine reported in secitific publication PMID: 32523000

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Biotin (Vitamin B7 or Vitamin H) Antibody (NBP2-34646IR)(1)

Publication using NBP2-34646IR

• Kumar S, Park H, Cho H et al. Overcoming evanescent field decay using 3D-tapered nanocavities for on-chip targeted molecular analysis Nat Commun Jun 10 2020 [PMID: 32523000] (ICC/IF, Bovine)