beta Galactosidase Antibody (13R4) [CoraFluor™ 1]

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Product Details

Summary
Reactivity EcSpecies Glossary
Applications WB, Flow, Ctrl, CyTOF-ready
Clone
13R4
Clonality
Monoclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

beta Galactosidase Antibody (13R4) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Beta-galactosidase.
Specificity
13R4 recognizes wild-type E. coli beta-galactosidase.
Isotype
IgG Lambda
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Control
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for beta Galactosidase Antibody (13R4) [CoraFluor™ 1]

  • EC 3.2.1.23; b-gal

Background

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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