Recombinant Human beta-1,3-Glucuronyltransferase 3/B3GAT3 Protein

Product Discontinued

Recombinant Human beta-1,3-Glucuronyltransferase 3/B3GAT3 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 29-335 of Human beta-1,3-Glucuronyltransferase 3/B3GAT3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSQPCDCLP PLRAAAEQLR QKDLRISQLQ AELRRPPPAP AQPPEPEALP TIYVVTPTYA RLVQKAELVR LSQTLSLVPR LHWLLVEDAE GPTPLVSGLL AASGLLFTHL VVLTPKAQRL REGEPGWVHP RGVEQRNKAL DWLRGRGGAV GGEKDPPPPG TQGVVYFADD DNTYSRELFE EMRWTRGVSV WPVGLVGGLR FEGPQVQDGR VVGFHTAWEP SRPFPVDMAG FAVALPLLLD KPNAQFDSTA PRGHLESSLL SHLVDPKDLE PRAANCTRVL VWHTRTEKPK MKQEEQLQRQ GRGSDPAIEV

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: B3GAT3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 36.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human beta-1,3-Glucuronyltransferase 3/B3GAT3 Protein

• B3GAT3
• beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
• Beta-1,3-glucuronyltransferase 3
• EC 2.4.1.135
• galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
• GLCATI
• GlcAT-I
• GlcUAT-I
• Glucuronosyltransferase I
• Sqv-8-like protein
• UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase

Background

Beta-1,3-glucuronyltransferase 3, also known as B3GAT3, is involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. This protein can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. It shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Recombinant human B3GAT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: B3GAT3