Human beta-1,3-Glucuronyltransferase 3/B3GAT3 ELISA Kit (Colorimetric)

ELISA: Human beta-1,3-Glucuronyltransferase 3/B3GAT3 ELISA Kit (Colorimetric) [NBP2-60545] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 3.75 - 240 ng/mL (example only; lot dependent)
• Sensitivity: 1.8 ng/mL (example only; lot dependent)

Human beta-1,3-Glucuronyltransferase 3/B3GAT3 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 3.75 - 240 ng/mL (example only; lot dependent)
• Sensitivity: 1.8 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10.4% (example only; lot dependent)
• Intra-Assay: %CV < 5.5% (example only; lot dependent)
• Spike Recovery: 91 - 114% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: B3GAT3

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human beta-1,3-Glucuronyltransferase 3/B3GAT3 ELISA Kit (Colorimetric)

• B3GAT3
• beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
• Beta-1,3-glucuronyltransferase 3
• EC 2.4.1.135
• galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
• GLCATI
• GlcAT-I
• GlcUAT-I
• Glucuronosyltransferase I
• Sqv-8-like protein
• UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase

Background

B3GAT3 is encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for beta-1,3-Glucuronyltransferase 3/B3GAT3 ELISA Kit (NBP2-60545). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: B3GAT3