![Flow Cytometry: Baculovirus Envelope gp64 protein Antibody (AcV1) [NBP2-00208] - Staining of baculovirus-infected insect cells with 0.25 ug of Mouse IgG2a kappa Isotype Control Purified (blue) or 0.25 ug of Anti-Baculovirus Envelope gp64 Protein Purified (purple) followed by F(ab')2 Anti-Mouse IgG PE . Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Baculovirus-Envelope-gp64-protein-Antibody-AcV1-Flow-Cytometry-NBP2-00208-img0001.jpg "Flow Cytometry: Baculovirus Envelope gp64 protein Antibody (AcV1) [NBP2-00208] - Staining of baculovirus-infected insect cells with 0.25 ug of Mouse IgG2a kappa Isotype Control Purified (blue) or 0.25 ug of Anti-Baculovirus Envelope gp64 Protein Purified (purple) followed by F(ab')2 Anti-Mouse IgG PE . Total viable cells were used for analysis.")
| Reactivity | V-ViSpecies Glossary |
| Applications | Flow |
| Clone | AcV1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.5 mg/ml |
| Description | The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV). The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection. The AcV1 antibody can be used in flow cytometry-based viral titration experiments. Purity: greater than 90%, as determined by SDS-PAGE. Affinity chromatography purified. |
| Immunogen | The immunogen for this antibody is baculovirus envelope gp64 protein. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The AcV1 antibody has been tested by flow cytometry on baculovirus infected insect cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.2) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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