B-Phycoerythrin Antibody - Azide and BSA Free

Western Blot: B-Phycoerythrin Antibody - Azide Free [NB120-7011] - Lane 1: B-Phycoerythrin reduced. Lane 2: B-Phycoerythrin reduced. Lane 3: none. Load: 1ug per lane. Primary antibody: Beta -Phycoerythrin antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: incubated with blocking buffer for Gli-2. Other band(s): none.

• Reactivity: All
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

B-Phycoerythrin Antibody - Azide and BSA Free Summary

• Description: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified B-Phycoerythrin [Porphyridium cruentum]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: B-Phycoerythrin [Porphyridium cruentum] (Uniprot: P11392)
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:1000-1:5000
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been tested by western blot and is suitable for dot blot, ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Reactivity Notes

Cross reactivity against B-Phycoerythrin from other sources is unknown

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Purity: Delipidation and Defibrination

Alternate Names for B-Phycoerythrin Antibody - Azide and BSA Free

• B-Phycoerythrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B-Phycoerythrin Antibody (NB120-7011)(5)

Publications using NB120-7011

• Tan HX, Juno JA, Esterbauer R et al. Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles Science immunology 2022-01-28 [PMID: 35089815] (IF/IHC)
• Ning J, Gavil NV, Wu S et al. Functional virus-specific memory T cells survey glioblastoma Cancer immunology, immunotherapy : CII 2022-01-10 [PMID: 35001153]
• Spencer DA, Quiniou SMA, Crider J et al. Insights into the dynamics of memory, effector and apoptotic cytotoxic T lymphocytes in channel catfish, Ictalurus punctatus Dev. Comp. Immunol. 2019-03-01 [PMID: 30447233]
• Mahmud SA, Manlove LS, Schmitz HM et al. Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells. Nat Immunol. [PMID: 24633226] (IHC-Fr)
  
  Details:
  Citation using the Azide Free form of this antibody.
• Schenkel JM, Fraser KA, Vezys V, Masopust D. Sensing and alarm function of resident memory CD8+ T cells. Nat Immunol. [PMID: 23542740] (IHC-Fr, ICC/IF)
  
  Details:
  Citation using the Azide Free form of this antibody.