Avi Epitope Tag Antibody (2623F) [Unconjugated]

Western blot shows recombinant human VEGF-165 Avi Tag protein and recombinant human OX40 His Tag protein (negative control). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for recombinant human VEGF-165 Avi Tag protein at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows recombinant OX40 Avi Tag protein, loaded at 0.2 mg/mL. A specific band was detected for recombinant OX40 Avi Tag at approximately 74 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: All-Multi
• Applications: WB, B/N
• Clone: 2623F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Avi Epitope Tag Antibody (2623F) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human OX40/TNFRSF4 Avi-tag
• Specificity: Detects proteins with Avi Tag in direct ELISAs and Western blots. In Western blots, no cross-reactivity with proteins with His Tag is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Neutralization 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Avi Epitope Tag Antibody (2623F) [Unconjugated]

• Avi Epitope Tag
• Avi Tag
• AviTag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Avi Epitope Tag Antibody (MAB10546)(1)

Publication using MAB10546

• Jim Baggen, Maarten Jacquemyn, Leentje Persoons, Els Vanstreels, Valerie E. Pye, Antoni G. Wrobel et al. TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry Cell 2023-08-03 [PMID: 37421949] (Surface Plasmon Resonance (SPR), N/A)