Asparaginase Antibody [Biotin]



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Asparaginase Antibody [Biotin] Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Asparaginase (E. coli).
Cytoplasmic and Secreted.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti Biotin, anti Rabbit Serum as well as purified and partially purified Asparaginase (from E. coli).
Delipidation and Defibrination
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
0.01% Sodium Azide
Delipidation and Defibrination


Application Notes
This product has been assayed against 1.0 ug of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:3,200 - 1:12,800 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross-reacts with E. coli.Not yet tested in other species.


Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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