APC-PCI Complex Antibody (M36) - BSA Free

ELISA: APC-PCI Complex Antibody (M36) - BSA Free [NBP2-41380] - The figure illustrates the calibration curve of a sandwich assay with NBP2-41380 as the capture antibody and biotinylated detection antibody.

• Reactivity: Hu
• Applications: ELISA
• Clone: M36
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

APC-PCI Complex Antibody (M36) - BSA Free Summary

• Immunogen: Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%).
• Epitope: The epitope is loctated in the calcium-binding N-terminal EGF domain of protein C.
• Specificity: Antibody is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
• Application Notes: ELISA: NBP2-41380 shows excellent reactivity towards PCI in cleaved and complex form in ELISA. NBP2-41380 is a part of the APC-PCI Matched Reagent Set, and can be used in ELISA as a capture antibody in combination with SA001RA as the detection antibody. (1-5) WB: NBP2-41380 shows low reactivity against denatured antigen in SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.