Reactivity | HuSpecies Glossary |
Applications | ELISA |
Clone | M36 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1 mg/ml |
Immunogen | Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%). |
Epitope | The epitope is loctated in the calcium-binding N-terminal EGF domain of protein C. |
Specificity | Antibody is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA: NBP2-41380 shows excellent reactivity towards PCI in cleaved and complex form in ELISA. NBP2-41380 is a part of the APC-PCI Matched Reagent Set, and can be used in ELISA as a capture antibody in combination with SA001RA as the detection antibody. (1-5) WB: NBP2-41380 shows low reactivity against denatured antigen in SDS-PAGE. |
Storage | Store at 4C in the dark. |
Buffer | 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl |
Preservative | 15mM Sodium Azide |
Concentration | 1 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NBP2-41380AF350 | |
Alexa Fluor 405 | NBP2-41380AF405 | |
Alexa Fluor 488 | NBP2-41380AF488 | |
Alexa Fluor 532 | NBP2-41380AF532 | |
Alexa Fluor 594 | NBP2-41380AF594 | |
Alexa Fluor 647 | NBP2-41380AF647 | |
Alexa Fluor 700 | NBP2-41380AF700 | |
Alexa Fluor 750 | NBP2-41380AF750 | |
Biotin | NBP2-41380B | |
DyLight 350 | NBP2-41380UV | |
DyLight 405 | NBP2-41380V | |
DyLight 488 | NBP2-41380G | |
DyLight 550 | NBP2-41380R | |
DyLight 594 | NBP2-41380DL594 | |
DyLight 650 | NBP2-41380C | |
DyLight 680 | NBP2-41380FR | |
DyLight 755 | NBP2-41380IR | |
HRP | NBP2-41380H |
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