![ELISA: APC-PCI Complex Antibody (M36) - BSA Free [NBP2-41380] - The figure illustrates the calibration curve of a sandwich assay with NBP2-41380 as the capture antibody and biotinylated detection antibody.](http://images.novusbio.com/fullsize/APC-PCI-Complex-Antibody-M36-BSA-Free-ELISA-NBP2-41380-img0001.jpg "ELISA: APC-PCI Complex Antibody (M36) - BSA Free [NBP2-41380] - The figure illustrates the calibration curve of a sandwich assay with NBP2-41380 as the capture antibody and biotinylated detection antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Clone | M36 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse APC-PCI Complex Antibody (M36) - BSA Free (NBP2-41380) is a monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%). |
| Epitope | The epitope is loctated in the calcium-binding N-terminal EGF domain of protein C. |
| Specificity | Antibody is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: NBP2-41380 shows excellent reactivity towards PCI in cleaved and complex form in ELISA. NBP2-41380 is a part of the APC-PCI Matched Reagent Set, and can be used in ELISA as a capture antibody in combination with SA001RA as the detection antibody. (1-5) WB: NBP2-41380 shows low reactivity against denatured antigen in SDS-PAGE. |
| Storage | Store at 4C in the dark. |
| Buffer | 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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