AKAP14 Antibody (SC0667)

Western Blot: AKAP14 Antibody (SC0667) [NBP2-67620] - Analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: A549

Immunocytochemistry/Immunofluorescence: AKAP14 Antibody (SC0667) [NBP2-67620] - Staining AKAP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: AKAP14 Antibody (SC0667) [NBP2-67620] - Staining AKAP14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: AKAP14 Antibody (SC0667) [NBP2-67620] - Staining AKAP14 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

AKAP14 Antibody (SC0667) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human AKAP14 aa 135-170 / 197. (SwissProt: Q86UN6 Human; Unigene: 471848 Mouse; Unigene: O35817 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AKAP14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AKAP14 Antibody (SC0667)

• A kinase (PRKA) anchor protein 14
• AKAP 28
• AKAP-14
• AKAP28Protein kinase A-anchoring protein 14
• A-kinase anchor protein 14
• A-kinase anchor protein 28 kDa
• A-kinase anchoring protein 28

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKAP14