![Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of African Swine Fever Virus p54 protein (0.2 ug/ml) by lateral flow assay. Capture : Biotin-labeled African Swine Fever Virus p54 antibody [GT1075] (3.32 ug/ml)._x000B_ Detection : FITC-labeled African Swine Fever Virus p54 antibody [GT853] (3.32 ug/ml).](http://images.novusbio.com/fullsize/African-Swine-Fever-Virus-p54-Antibody-GT853-Western-Blot-NBP3-13671-img0006.jpg "Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of African Swine Fever Virus p54 protein (0.2 ug/ml) by lateral flow assay. Capture : Biotin-labeled African Swine Fever Virus p54 antibody [GT1075] (3.32 ug/ml)._x000B_ Detection : FITC-labeled African Swine Fever Virus p54 antibody [GT853] (3.32 ug/ml).")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, LFA |
| Clone | GT853 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of African Swine Fever Virus p54 (ASFV (strain: BA71V)). The exact sequence is proprietary. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | E183L |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | E183L |
![Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - African Swine Fever Virus p54 antibody [GT853] detects African Swine Fever Virus p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/African-Swine-Fever-Virus-p54-Antibody-GT853-Immunocytochemistry-Immunofluorescence-NBP3-13671-img0002.jpg "Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - African Swine Fever Virus p54 antibody [GT853] detects African Swine Fever Virus p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:1000. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - African Swine Fever Virus p54 antibody [GT853] detects African Swine Fever Virus p54 protein at cytoplasm by immunohistochemical analysis. Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/African-Swine-Fever-Virus-p54-Antibody-GT853-Immunohistochemistry-Paraffin-NBP3-13671-img0003.jpg "Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - African Swine Fever Virus p54 antibody [GT853] detects African Swine Fever Virus p54 protein at cytoplasm by immunohistochemical analysis. Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/African-Swine-Fever-Virus-p54-Antibody-GT853-Western-Blot-NBP3-13671-img0001.jpg "Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Sandwich ELISA: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Sandwich ELISA detection of recombinant African Swine Fever Virus p54 using antibodies as below. Capture: African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) (5 ug/mL) Detection: HRP-conjugated African Swine Fever Virus p54 antibody [GT1075] (NBP3-13672) (1 ug/mL) Please notice that NBP3-13672 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13671. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/African-Swine-Fever-Virus-p54-Antibody-GT853-Sandwich-ELISA-NBP3-13671-img0004.jpg "Sandwich ELISA: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Sandwich ELISA detection of recombinant African Swine Fever Virus p54 using antibodies as below. Capture: African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) (5 ug/mL) Detection: HRP-conjugated African Swine Fever Virus p54 antibody [GT1075] (NBP3-13672) (1 ug/mL) Please notice that NBP3-13672 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13671. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Sandwich ELISA detection of recombinant African Swine Fever Virus p54 using antibodies as below. Capture: African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) (5 ug/mL) Detection: African Swine Fever Virus p54 antibody (NBP3-13435) (1 ug/mL).](http://images.novusbio.com/fullsize/African-Swine-Fever-Virus-p54-Antibody-GT853-Sandwich-ELISA-NBP3-13671-img0005.jpg "Sandwich ELISA: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Sandwich ELISA detection of recombinant African Swine Fever Virus p54 using antibodies as below. Capture: African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) (5 ug/mL) Detection: African Swine Fever Virus p54 antibody (NBP3-13435) (1 ug/mL).")
![Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of HEK293 expressed, African Swine Fever Virus p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: African Swine Fever Virus p54 antibody (NBP3-13671 [GT853]) Detection: African Swine Fever Virus p54 antibody Samples (40 ng) : 1. African Swine Fever Virus p54 (ECD) protein 2. Lysis buffer](http://images.novusbio.com/fullsize/nbp3-13671_mouse-african-swine-fever-virus-p54-mab-gt853-91020242001069.jpg "Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of HEK293 expressed, African Swine Fever Virus p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: African Swine Fever Virus p54 antibody (NBP3-13671 [GT853]) Detection: African Swine Fever Virus p54 antibody Samples (40 ng) : 1. African Swine Fever Virus p54 (ECD) protein 2. Lysis buffer")
![Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of HEK293 expressed, African Swine Fever Virus p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: African Swine Fever Virus p54 antibody (NBP3-13671 [GT853]) Detection: African Swine Fever Virus p54 antibody Samples (40 ng) : 1. African Swine Fever Virus p54 (ECD) protein 2. Lysis buffer](http://images.novusbio.com/fullsize/nbp3-13671_mouse-african-swine-fever-virus-p54-mab-gt853-910202420131248.jpg "Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of HEK293 expressed, African Swine Fever Virus p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: African Swine Fever Virus p54 antibody (NBP3-13671 [GT853]) Detection: African Swine Fever Virus p54 antibody Samples (40 ng) : 1. African Swine Fever Virus p54 (ECD) protein 2. Lysis buffer")
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")