Aducanumab Antibody (2374E) [Unconjugated]

Western blot shows Brentuximab (negative control), Aducanumab and Ipilimumab (negative control) biosimilars. PVDF membrane was probed with 2 µg/mL of Anti-Aducanumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Aducanumab at approximately 250 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: All-Multi
• Applications: WB
• Clone: 2374E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Aducanumab Antibody (2374E) [Unconjugated] Summary

• Immunogen: Full length Aducanumab
• Specificity: Detects human Aducanumab in direct ELISAs and Western blots. In Western blots, no cross-reactivity with Brentuximab and Ipilimumab is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aducanumab Antibody (2374E) [Unconjugated]

• Aducanumab

Background

The anti-Aducanumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Aducanumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.