Adenovirus Antibody (8C4)

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Product Details

Summary
Reactivity V, V-ViSpecies Glossary
Applications ELISA, Func, ICC/IF, IHC, B/N, LFA
Clone
8C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Adenovirus Antibody (8C4) Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human adenovirus type 1 and canine adenovirus type 1.
Specificity
Hexon antigen of human adenovirus
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunodiffusion
  • Immunohistochemistry 1:10-1:500
  • Lateral Flow Assay
  • Sandwich ELISA 1:100-1:2000
Application Notes
Recommended pair for sandwich ELISA (capture-detection): NB600-413 - NB200-425. This antibody has been reported in the literature to work in Immunohistochemistry-Frozen sections (PMID: 19622582). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26861248). Use in Block/Neutralize reported in scientific literature (PMID:31848338). This antibody is CyTOF ready.
Control
CXADR Overexpression Lysate
Publications
Read Publications using
NB600-413 in the following applications:

  • B/N
    1 publication
  • 2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Adenovirus Antibody (8C4)

  • Adenovirus

Background

Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterized by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenovirus Antibody (NB600-413)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Cynomolgus Monkey, Virus.

We have publications tested in 3 applications: B/N, ICC/IF, WB.


Filter By Application
B/N
(1)
ICC/IF
(2)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Cynomolgus Monkey
(1)
Virus
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB600-413 Applications Species
Boilesen DR, Neckermann P, Willert T et al. Efficacy and synergy with cisplatin of an adenovirus vectored therapeutic E1E2E6E7 vaccine against HPV genome positive C3 cancers in mice Cancer immunology research 2022-12-19 [PMID: 36534088]
Hassan AO, Kafai NM, Dmitriev IP et al. A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2 Cell 2020-10-01 [PMID: 32931734]
Fusciello M, Fontana F, TAhtinen S, et al. Artificially cloaked viral nanovaccine for cancer immunotherapy Nat Commun 2019-12-17 [PMID: 31848338] (B/N, Mouse) B/N Mouse
Kuryk L, Moller AW, Vuolanto A et al. Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks Int J Mol Sci 2019-01-31 [PMID: 30709038] (ICC/IF, Human) ICC/IF Human
Ragonnaud E, Schroedel S, Mariya S et al. Replication deficient human adenovirus vector serotype 19a/64: Immunogenicity in mice and female cynomolgus macaques. Vaccine 2018-10-01 [PMID: 30190120] (Mouse, Cynomolgus Monkey) Mouse, Cynomolgus Monkey
Garofalo, M, Saari, H Et al. Antitumor effect of oncolytic virus and paclitaxel encapsulated in extracellular vesicles for lung cancer treatment. J Control Release 2018-08-10 [PMID: 29864473] (WB) WB
Garofalo M, Iovine B, Kuryk L et al. Oncolytic adenovirus loaded with L-carnosine as novel strategy to enhance the anti-tumor activity Mol. Cancer Ther. 2016-02-09 [PMID: 26861248] (ICC/IF, Virus) ICC/IF Virus
Fernes MS, Gomes EM, Butcher LD et al. Growth Inhibition of Human Multiple Myeloma Cells by an Oncolytic Adenovirus Carrying the CD40 Lig Transgene. Clin Cancer Res;15(15):4847-4856. 2009-01-01 [PMID: 19622582]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Adenovirus Antibody (NB600-413). (Showing 1 - 1 of 1 FAQs).

  1. What was the serotype of the immunogen used for the 8C4 antibody?
    • As an immunogen for NB600-413 we used both human adenovirus type 1 and canine adenovirus type 1 as an immunogen for this antibody.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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