ADAM32 Antibody

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ADAM32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAM32 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ADAM32 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
  Ser17-Thr476
  Accession # Q8TC27
• Specificity: Detects human ADAM32 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ADAM9, rhADAM22, and rhADAM23 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ADAM32
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM32 Antibody

• a disintegrin and metalloprotease domain 32
• a disintegrin and metalloproteinase domain 32
• ADAM 32
• ADAM metallopeptidase domain 32
• ADAM32
• disintegrin and metalloproteinase domain-containing protein 32
• FLJ26299
• FLJ29004
• metalloproteinase 12-like protein

Background

ADAM32 (a disintegrin and metalloprotease domain 32) is a 90-100 kDa member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771 amino acid (aa) type I transmembrane protein. It contains a 158 aa proregion (aa 17-174) and a 508 aa extracellular domain (ECD) (aa 175-682). The ECD contains a nonfunctional metalloprotease domain (aa 186-383), an integrin-binding disintegrin region (aa 391-479), a Cys-rich segment (aa 480 - 502) and an EGF-like domain (aa 622-654). In the testis, mature ADAM32 is approximately 98 kDa in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44 kDa product. There are two potential splice events that show a deletion of aa 306-401 plus a 53 aa substitution for the N-terminal 46 amino acids. Over aa 17-476, human ADAM32 is 66% aa identical to mouse ADAM32.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAM32
• Uniprot:
  • Q8TC27()