ADAM32 Antibody (539220) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 539220
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

ADAM32 Antibody (539220) [Alexa Fluor® 594] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
  Ser17-Thr476
  Accession # Q8TC27
• Specificity: Detects human ADAM32 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM32 Antibody (539220) [Alexa Fluor® 594]

• a disintegrin and metalloprotease domain 32
• a disintegrin and metalloproteinase domain 32
• ADAM 32
• ADAM metallopeptidase domain 32
• ADAM32
• disintegrin and metalloproteinase domain-containing protein 32
• FLJ26299
• FLJ29004
• metalloproteinase 12-like protein

Background

ADAM32 (a disintegrin and metalloprotease domain 32) is a 98 kDa member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771 amino acid (aa) type I transmembrane protein. It contains a 158 aa proregion (aa 17‑174) and a 508 aa extracellular domain (ECD) (aa 175‑682). The ECD contains a nonfunctional metalloprotease domain (aa 186‑383), an integrin‑binding disintegrin region (aa 391‑479), a Cys‑rich segment (aa 480‑502) and an EGF‑like domain (aa 622‑654). In the testis, mature ADAM32 is 98 kDa in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44 kDa product. There are two potential splice events that show a deletion of aa 306‑401 plus a 53 aa substitution for the N‑terminal 46 amino acids. Over aa 17‑476, human ADAM32 is 66% aa identical to mouse ADAM32.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.