ACSF2 Antibody (OTI6E4)

Immunocytochemistry/Immunofluorescence: ACSF2 Antibody (OTI6E4) [NBP2-02027] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACSF2.

Immunohistochemistry-Paraffin: ACSF2 Antibody (OTI6E4) [NBP2-02027] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ACSF2 mouse monoclonal antibody.

Flow Cytometry: ACSF2 Antibody (OTI6E4) [NBP2-02027] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACSF2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: ACSF2 Antibody (OTI6E4) [NBP2-02027] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACSF2 mouse monoclonal antibody.

Immunoprecipitation: ACSF2 Antibody (OTI6E4) [NBP2-02027] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ACSF2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: OTI6E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.53 mg/ml

ACSF2 Antibody (OTI6E4) Summary

• Description: Novus Biologicals Mouse ACSF2 Antibody (OTI6E4) (NBP2-02027) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ACSF2(NP_079425) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACSF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Immunoprecipitation
  • Western Blot
• Theoretical MW: 67.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.53 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACSF2 Antibody (OTI6E4)

• ACSMW
• acyl-CoA synthetase family member 2
• AVYV493
• EC 6.2.1
• EC 6.2.1.-
• EC 6.2.1.26
• mitochondrial
• PPARG binding, long chain fatty acid acyl Co-A ligase like

Background

Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA.Has some preference toward medium-chain substrates. Plays a role in adipodyte differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACSF2
• Entrez:
  • 80221(Human)
• Uniprot:
  • Q96CM8(Human)