A2B5 Antibody (105) [Unconjugated]

NTera-2 human testicular embryonic carcinoma cell line either treated with 10 µM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

A2B5 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days) using Mouse Anti-Human/Mouse/Rat/ Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

A2B5 Antibody (105) [Unconjugated] Summary

• Immunogen: Embryonic chicken retinal cells
• Specificity: Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1-4).
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A2B5 Antibody (105) [Unconjugated]

• A2B5 marker
• A2B5

Background

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A2B5 Antibody (MAB1416)(27)

Publications using MAB1416

• Takasugi, M;Ohtani, N;Takemura, K;Emmrich, S;Zakusilo, FT;Yoshida, Y;Kutsukake, N;Mariani, JN;Windrem, MS;Chandler-Militello, D;Goldman, SA;Satoh, J;Ito, S;Seluanov, A;Gorbunova, V; CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance Cell reports 2023-09-13 [PMID: 37708026] (Flow Cytometry, Human)
• Dou Ye, Suqing Qu, Yinxiang Yang, Zhaoyan Wang, Qian Wang, Weipeng Liu et al. Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression iScience 5/19/2023 [PMID: 37168574]
• Ye D, Wang Q, Yang Y et al. Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes Cellular and molecular neurobiology 12/22/2022 [PMID: 36547781]
• Titus HE, Xu H, Robinson AP et al. Repurposing the cardiac glycoside digoxin to stimulate myelin regeneration in chemically-induced and immune-mediated mouse models of multiple sclerosis Glia 7/9/2022 [PMID: 35809238]
• Haipeng Zhou, Ying He, Zhaoyan Wang, Qian Wang, Caiyan Hu, Xiaohua Wang et al. Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development Journal of Translational Medicine 12/1/2021 [PMID: 33933125]
• KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler Donor cell memory confers a metastable state of directly converted cells Cell Stem Cell, 2021-04-12;0(0):. 2021-04-12 [PMID: 33848472] (ICC, Mouse)
• M Yazdankhah, S Ghosh, P Shang, N Stepicheva, S Hose, H Liu, X Chamling, S Tian, MLG Sullivan, MJ Calderon, CS Fitting, J Weiss, A Jayagopal, JT Handa, JA Sahel, JS Zigler, PR Kinchingto, DJ Zack, D Sinha BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes Autophagy, 2021-01-19;0(0):1-20. 2021-01-19 [PMID: 33404293] (ICC, IHC, Rat)
• JP Zepecki, KM Snyder, MM Moreno, E Fajardo, A Fiser, J Ness, A Sarkar, SA Toms, N Tapinos Regulation of human glioma cell migration, tumor growth, and stemness gene expression using a Lck targeted inhibitor Oncogene, 2018-10-23;0(0):. 2018-10-23 [PMID: 30353164] (IHC, Rat)
• JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency Cell Rep, 2017-04-04;19(1):20-35. 2017-04-04 [PMID: 28380358] (Flow Cytometry, Human)
• MY Cha, YW Kwon, HS Ahn, H Jeong, YY Lee, M Moon, SH Baik, DK Kim, H Song, EC Yi, D Hwang, HS Kim, I Mook-Jung Protein-Induced Pluripotent Stem Cells Ameliorate Cognitive Dysfunction and Reduce A? Deposition in a Mouse Model of Alzheimer's Disease Stem Cells Transl Med, 2016-08-15;6(1):293-305. 2016-08-15 [PMID: 28170178] (ICC, Mouse)
• Xiaoyang Li, Alicia Aleardi, Jue Wang, Yang Zhou, Rodrigo Andrade, Zhengqing Hu Differentiation of Spiral Ganglion-Derived Neural Stem Cells into Functional Synaptogenetic Neurons Stem Cells and Development 5/15/2016 [PMID: 27021700]
• Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration. J Neurosci, 2015-01-07;35(1):4-20. 2015-01-07 [PMID: 25568099] (IHC-Fr, Mouse)
• Leite C, Silva N, Mendes S, Ribeiro A, de Faria J, Lourenco T, dos Santos F, Andrade P, Cardoso C, Vieira M, Paiva A, da Silva C, Cabral J, Relvas J, Graos M Differentiation of human umbilical cord matrix mesenchymal stem cells into neural-like progenitor cells and maturation into an oligodendroglial-like lineage. PLoS ONE, 2014-10-30;9(10):e111059. 2014-10-30 [PMID: 25357129] (IHC-Fr, Mouse)
• Robinson A, Rodgers J, Goings G, Miller S Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis. PLoS ONE, 2014-09-23;9(9):e107649. 2014-09-23 [PMID: 25247590] (Flow Cytometry, Mouse)
• Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S Generation of neuronal progenitor cells in response to tumors in the human brain. Stem Cells, 2014-01-01;32(1):244-57. 2014-01-01 [PMID: 24170295] (IHC, Human)
• Najm , Fadi J, Lager , Angela M, Zaremba , Anita, Wyatt , Krysta, Caprariello , Andrew V, Factor , Daniel C, Karl , Robert T, Maeda , Tadao, Miller , Robert H, Tesar , Paul J Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol, 2013-04-14;31(5):426-33. 2013-04-14 [PMID: 23584611] (Flow Cytometry, Mouse)
• Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M In vivo generation of neural tumors from neoplastic pluripotent stem cells models early human pediatric brain tumor formation. Stem Cells, 2012-03-01;30(3):392-404. 2012-03-01 [PMID: 22213600] (Flow Cytometry, ICC, Human)
• Piotr Walczak, Angelo H. All, Nidhi Rumpal, Michael Gorelik, Heechul Kim, Anil Maybhate et al. Human glial-restricted progenitors survive, proliferate, and preserve electrophysiological function in rats with focal inflammatory spinal cord demyelination Glia 3/1/2011 [PMID: 21264955]
• Seshadri S, Kamiya A, Yokota Y, Prikulis I, Kano S, Hayashi-Takagi A, Stanco A, Eom TY, Rao S, Ishizuka K, Wong P, Korth C, Anton ES, Sawa A Disrupted-in-Schizophrenia-1 expression is regulated by beta-site amyloid precursor protein cleaving enzyme-1-neuregulin cascade. Proc. Natl. Acad. Sci. U.S.A., 2010-03-08;107(12):5622-7. 2010-03-08 [PMID: 20212127] (ICC, Human)
• Todorich B, Zhang X, Slagle-Webb B, Seaman WE, Connor JR Tim-2 is the receptor for H-ferritin on oligodendrocytes. J. Neurochem., 2008-11-05;107(6):1495-505. 2008-11-05 [PMID: 19014383] (ICC, Rat)
• Dobson NR, Zhou YX, Flint NC, Armstrong RC Musashi1 RNA-binding protein regulates oligodendrocyte lineage cell differentiation and survival. Glia, 2008-02-01;56(3):318-30. 2008-02-01 [PMID: 18098125] (ICC, Rat)
• Chen H, Kintner DB, Jones M, Matsuda T, Baba A, Kiedrowski L, Sun D AMPA-mediated excitotoxicity in oligodendrocytes: role for Na(+)-K(+)-Cl(-) co-transport and reversal of Na(+)/Ca(2+) exchanger. J. Neurochem., 2007-05-08;102(6):1783-95. 2007-05-08 [PMID: 17490438] (ICC, Rat)
• Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW Efficient induction of oligodendrocytes from human embryonic stem cells. Stem Cells, 2006-10-19;25(2):419-24. 2006-10-19 [PMID: 17053214] (ICC, Human)
• Lee DS, Yu K, Rho JY, Lee E, Han JS, Koo DB, Cho YS, Lee KK, Han YM Cyclopamine treatment of human embryonic stem cells followed by culture in human astrocyte medium promotes differentiation into nestin- and GFAP-expressing astrocytic lineage. Life Sci., 2006-09-23;80(2):154-9. 2006-09-23 [PMID: 17028036] (ICC, Human)
• Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Brain Res. Dev. Brain Res., 2004-09-17;152(2):159-69. 2004-09-17 [PMID: 15351504] (ICC, Human)
• Rosin C, Bates TE, Skaper SD Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms. J. Neurochem., 2004-09-01;90(5):1173-85. 2004-09-01 [PMID: 15312172] (ICC, Human)
• Tsai HH, Tessier-Lavigne M, Miller RH Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Development, 2003-05-01;130(10):2095-105. 2003-05-01 [PMID: 12668624] (ICC, Chicken, Rat)