5-MethylCytosine ELISA Kit (Colorimetric)

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - The workflow for the 5-Methylcytosine DNA ELISA kit utilizes the indirect ELISA methodology where denatured, single-stranded DNA (ssDNA) samples are coated on the plate well surfaces and a 5-mC mAb and conjugate HRP-Ab are added to the wells. Indirect det

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - Comparison of genomic DNA Mass Spectroscopy versus 5-MethylCytosine ELISA analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - A typical standard curve for 5-MethylCytosine ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: DNA samples
• Standard Curve Range: 5-100% (100 ng/ul
• Sensitivity: ~0.5% 5-methylcytosine per 100ng single-stranded DNA

5-MethylCytosine ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 5-100% (100 ng/ul
• Sensitivity: ~0.5% 5-methylcytosine per 100ng single-stranded DNA
• Assay Type: Indirect ELISA
• Inter-Assay: N/A
• Intra-Assay: N/A
• Spike Recovery: N/A
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for 5-MethylCytosine ELISA Kit (Colorimetric)

• 5-mC
• 5-Methyl Cytosine
• Cytosine (5-Methyl)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.