5-MethylCytosine ELISA Kit (Colorimetric)

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - The workflow for the 5-Methylcytosine DNA ELISA kit utilizes the indirect ELISA methodology where denatured, single-stranded DNA (ssDNA) samples are coated on the plate well surfaces and a 5-mC mAb and conjugate HRP-Ab are added to the wells. Indirect det

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - Comparison of genomic DNA Mass Spectroscopy versus 5-MethylCytosine ELISA analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - A typical standard curve for 5-MethylCytosine ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: DNA samples
• Standard Curve Range: 5-100% (100 ng/ul
• Sensitivity: ~0.5% 5-methylcytosine per 100ng single-stranded DNA

5-MethylCytosine ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 5-100% (100 ng/ul
• Sensitivity: ~0.5% 5-methylcytosine per 100ng single-stranded DNA
• Assay Type: Indirect ELISA
• Inter-Assay: N/A
• Intra-Assay: N/A
• Spike Recovery: N/A
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for 5-MethylCytosine ELISA Kit (Colorimetric)

• 5-mC
• 5-Methyl Cytosine
• Cytosine (5-Methyl)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for 5-MethylCytosine ELISA Kit (NBP2-62131). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.