4-Hydroxynonenal ELISA Kit (Colorimetric)

ELISA: Universal 4-Hydroxynonenal ELISA Kit (Colorimetric) [NBP2-66364] - Samples were spiked with high concentrations of 4-Hydroxynonenal and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Universal 4-Hydroxynonenal ELISA Kit (Colorimetric) [NBP2-66364] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL

4-Hydroxynonenal ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: %CV < 4.52
• Intra-Assay: %CV < 4.1
• Spike Recovery: 89-107%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for 4-Hydroxynonenal ELISA Kit (Colorimetric)

• (2E)-4-Hydroxy-2-nonenal
• 2-Nonenal, 4-hydroxy-, (2E,4R)-
• 4 HNE
• 4Hydroxynonenal
• 4-Hydroxynonenal
• 4-hydroxy-trans-2-nonenal
• HNE

Background

4-HNE is a hydroxyalkenal which is produced by lipid peroxidation in cells. It is found in higher quantities during stress, due to the increase in the lipid peroxidation reaction that accompanies stress events. 4-Hydroxynonenanal is believed to play a key role in signal transduction in a number of pathways, including cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for 4-Hydroxynonenal ELISA Kit (NBP2-66364)(3)

Publications using NBP2-66364

• Miriam Ferrer, Nicholas Mourikis, Emma E. Davidson, Sam O. Kleeman, Marta Zaccaria, Jill Habel, Rachel Rubino, Qing Gao, Thomas R. Flint, Lisa Young, Claire M. Connell, Michael J. Lukey, Marcus D. Goncalves, Eileen P. White, Ashok R. Venkitaraman, Tobias Janowitz Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia Cell metabolism 2024-04-23 [PMID: 37311455]
• Herrock O, Deer E, Amaral LM et al. Inhibiting B cell activating factor attenuates preeclamptic symptoms in placental ischemic rats American journal of reproductive immunology (New York, N.Y. : 1989) 2023-02-16 [PMID: 36794639]
• Miller L, Thompson K, Pavlenco C Et al. The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized Controlled Trial Nutrients 2021-10-15 [PMID: 34684621]