4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free

Dot Blot: 4-Hydroxy-2-hexenal Antibody (5C11.1) [NBP3-18263] - Dot blot analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 5C11.1 (NBP3-18263). Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody (NBP3-18263) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.

• Reactivity: All-NA
• Applications: WB, DB, ELISA
• Clone: 5C11.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free Summary

• Immunogen: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
• Specificity: Specific for 4-HHE. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:1000
  • Western Blot 1:1000
• Application Notes: Optimal dilutions for assays should be determined by the user.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.4, 50% glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for 4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free

• 4-HHE;4-hydroxy Hexenal;4-Hydroxy-2-hexenal;4-Hydroxy-2-hexenal-modified;HHE;MG;MG-modified protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.