4-amino Biphenyl DNA Antibody (4C11)

Immunohistochemistry: 4-amino Biphenyl DNA Antibody (4C11) [NB100-415] - Human breast tumor tissue with high (A) and low (B) adduct levels. Images courtesy of Dr. Regina Santella.

• Reactivity: Mu, All
• Applications: ELISA, ICC/IF, IHC
• Clone: 4C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

4-amino Biphenyl DNA Antibody (4C11) Summary

• Immunogen: 4-amino Biphenyl DNA
• Localization: Nuclear
• Clonality: Monoclonal
• Host: Mouse
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50 1:100
  • Immunohistochemistry 1:50-1:100
• Application Notes: This 4-amino Biphenyl DNA (4C11) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry.

Reactivity Notes

Detects DNA from all species Mouse reactivity reported in scientific literature (PMID: 12700401).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for 4-amino Biphenyl DNA Antibody (4C11)

• 4-AMBP DNA
• 4-aminobiphenyl DNA
• 4-amino-Biphenyl DNA

Background

4-amino Biphenyl (4-ABP) is an arylamine (aromatic amine) compound and as one of the major carcinogenic constituents of tobacco smoke other environmental sources, it poses critical health hazards to human beings. Under the action of carcinogen activating biotransformation enzymes such as cytochrome P4501A2, 4-ABP gets metabolized to generate a direct-acting mutagen known as N-hydroxy-aminobiphenyl. Nevertheless, the accumulation of biologically active metabolites is dependent on the balance between activation and detoxification enzymes. 4-ABP has been linked to bladder and breast cancers in human beings as well as in rodent models. Our 4-amino Biphenyl DNA antibody (4C11) is a monoclonal antiserum which recognizes 4-aminobiphenyl (4-ABP)-DNA adducts and it has been characterized by competitive enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC) and Immunocytochemistry/Immunofluorescence (ICC/IF) techniques. This antibody does not recognize the DNA adducts of several other aromatic amines tested including 1-aminopyrene, 8-nitro-1-aminopyrene, and 6-nitro-1-aminopyrene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4-amino Biphenyl DNA Antibody (NB100-415)(4)

Publications using NB100-415

• Faraglia, B et al. Evaluation of 4-aminobiphenyl-DNA adducts in human breast cancer: the influence of tobacco smoke. Carcinogenesis;24(4):719-25. 2003-04-01 [PMID: 12727801]
• McQueen, CA et al. Neonatal ontogeny of murine arylamine N-acetyltransferases: implications for arylamine genotoxicity. Toxicol Sci;73(2):279-86. 2003-06-01 [PMID: 12700401] (IHC, Mouse)
• al-Atrash, J et al. Quantitative immunohistochemical analysis of 4-aminobiphenyl-DNA in cultured cells and mice: comparison to gas chromatography/mass spectroscopy analysis. Chem Res Toxicol;8(5):747-52. 1995-07-01 [PMID: 7548758]
• Brown, A et al. Robust DNA Repair May Lower Breast Cancer Risk. Journal of the National Cancer Institute. 2005-01-01