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![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (top panel) or treated with with 5 ug/mL PHA (bottom panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002) (data not shown).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0001.jpg "Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (top panel) or treated with with 5 ug/mL PHA (bottom panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002) (data not shown).")
![Western Blot: LAG-3 Antibody Pack [NBP3-11743] - Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated or treated (+) with 1 ug/mL PHA for 5 days and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 ug/mL of Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for LAG-3 at approximately 60-75 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Western-Blot-NBP3-11743-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (bottom panel) or treated with with 5 ug/mL PHA (top panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23195) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0003.jpg)
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Analysis using the FITC conjugate of NBP1-97657. Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using LAG-3 (human), mAb (17B4).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Parthanatos Antibody Pack [NBP3-20191] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody [NB100-56599] (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nbp3-20191_parthanatos-antibody-pack-immunocytochemistry-immunofluorescence-352023143038..jpg "Immunocytochemistry/Immunofluorescence: Parthanatos Antibody Pack [NBP3-20191] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody [NB100-56599] (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: Parthanatos Antibody Pack [NBP3-20191] - Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, CTLL-2 mouse cytotoxic T cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat AIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1457) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for AIF at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.](http://images.novusbio.com/fullsize/nbp3-20191_parthanatos-antibody-pack-western-blot-35202314397..jpg)
![Western Blot: Parthanatos Antibody Pack [NBP3-20191] - Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nbp3-20191_parthanatos-antibody-pack-western-blot-352023144221..jpg)
