ZEB1 Antibodies
We offer ZEB1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Negative), Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each ZEB1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ZEB1 Antibodies can be used in a variety of model species: Canine, Human, Monkey, Mouse, Primate, Rat. Use the list below to choose the ZEB1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ZEB1 polyclonal antibodies and browse our ZEB1 monoclonal antibody catalog.
Alternate Names for ZEB1 Antibodies
anti-ZEB1 antibody, anti-AREB6MGC133261 antibody, anti-delta-crystallin enhancer binding factor 1 antibody, anti-DELTAEF1 antibody, anti-FECD6 antibody, anti-Negative regulator of IL2 antibody, anti-NIL-2-A antibody, anti-NIL-2-A zinc finger protein antibody, anti-NIL2A antibody, anti-posterior polymorphous corneal dystrophy 3 antibody, anti-PPCD3 antibody, anti-TCF-8 antibody, anti-TCF8BZP antibody, anti-transcription factor 8 (represses interleukin 2 expression) antibody, anti-Transcription factor 8 antibody, anti-ZEB antibody, anti-ZFHEP antibody, anti-ZFHX1A antibody, anti-zinc finger E-box binding homeobox 1 antibody, anti-zinc finger E-box-binding homeobox 1 antibody, anti-zinc finger homeodomain enhancer-binding protein antibody
26 Results for "zeb1" in Primary Antibodies
HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="ZEB1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB11504) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="ZEB1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # ![Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-298202414262614.jpg "Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Flow cytometric analysis of Jurkat cells labeling ZEB1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-2920241645327.jpg)
![Western Blot: ZEB1 Antibody (JE57-64) [NBP3-33111] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/500 dilution.<br/><br/> Lane 1: Hela cell lysate<br/> Lane 2: HUVEC cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 124 kDa<br/> Observed band size: 200 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33111) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33111_rabbit-zeb1-mab-je57-64-288202414191510.jpg "Western Blot: ZEB1 Antibody (JE57-64) [NBP3-33111] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/500 dilution.<br/><br/> Lane 1: Hela cell lysate<br/> Lane 2: HUVEC cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 124 kDa<br/> Observed band size: 200 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33111) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (JE57-64) [NBP3-33111] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33111) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33111_rabbit-zeb1-mab-je57-64-29820241492615.jpg)
![Western blot analysis on different lysates with NBP3-33111 at 1/500 dilution. Lane 1: HUVEC cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33111 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33111_-western-blot-639204594554721976.jpg)
![Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunoprecipitation-NBP3-14694-img0001.jpg "Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.")
![Western Blot: ZEB1 Antibody (SR1312) [NBP3-21854] - Western blot analysis using NBP3-21854 in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21854_rabbit-zeb1-mab-sr1312-5720231754860.jpg "Western Blot: ZEB1 Antibody (SR1312) [NBP3-21854] - Western blot analysis using NBP3-21854 in HEK293 cell lysate.")
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # 
