YTHD1 Antibodies
We offer YTHD1 Antibodies for use in common research applications: Western Blot. Each YTHD1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our YTHD1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the YTHD1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our YTHD1 polyclonal antibodies.
Alternate Names for YTHD1 Antibodies
anti-YTHD1 antibody, anti-YTHDF1 antibody, anti-C20orf21 antibody, anti-DACA-1 antibody, anti-Dermatomyositis associated with cancer putative autoantigen 1 antibody, anti-FLJ20391 antibody, anti-YTH domain family 1 antibody, anti-YTH domain family protein 1 antibody, anti-YTH domain family, member 1 antibody
6 Results for "ythd1" in Primary Antibodies
![Western Blot: YTHD1 Antibody (PSH0-23) [NBP3-33109] - Western blot analysis of YTHD1 on different lysates with Rabbit anti-YTHD1 antibody (NBP3-33109) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: MCF-7 cell lysate (10 ug/Lane) Lane 5: NIH/3T3 cell lysate (10 ug/Lane) Lane 6: PC-12 cell lysate (10 ug/Lane) Lane 7: HCT116 cell lysate (10 ug/Lane) Lane 8: Mouse testis liver tissue lysate (20 ug/Lane) Lane 9: Rat testis tissue lysate (20 ug/Lane) Lane 10: Mouse brain tissue lysate (20 ug/Lane) Lane 11: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 61 kDa Observed band size: 69 kDa Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33109) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33109_rabbit-ythd1-mab-psh0-23-288202414191533.jpg "Western Blot: YTHD1 Antibody (PSH0-23) [NBP3-33109] - Western blot analysis of YTHD1 on different lysates with Rabbit anti-YTHD1 antibody (NBP3-33109) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: MCF-7 cell lysate (10 ug/Lane) Lane 5: NIH/3T3 cell lysate (10 ug/Lane) Lane 6: PC-12 cell lysate (10 ug/Lane) Lane 7: HCT116 cell lysate (10 ug/Lane) Lane 8: Mouse testis liver tissue lysate (20 ug/Lane) Lane 9: Rat testis tissue lysate (20 ug/Lane) Lane 10: Mouse brain tissue lysate (20 ug/Lane) Lane 11: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 61 kDa Observed band size: 69 kDa Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33109) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: YTHD1 Antibody (PSH0-23) [NBP3-33109] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-YTHD1 antibody (NBP3-33109) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33109_rabbit-ythd1-mab-psh0-23-298202413585531.jpg)
![Flow Cytometry: YTHD1 Antibody (PSH0-23) [NBP3-33109] - Flow cytometric analysis of 293 cells labeling YTHD1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33109, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33109_rabbit-ythd1-mab-psh0-23-392024884667.jpg)
![Western Blot: YTHD1 Antibody [NBP3-33108] - Western blot analysis of YTHD1 on different lysates with Rabbit anti-YTHD1 antibody (NBP3-33108) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33108) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33108_rabbit-ythd1-pab-288202414191532.jpg "Western Blot: YTHD1 Antibody [NBP3-33108] - Western blot analysis of YTHD1 on different lysates with Rabbit anti-YTHD1 antibody (NBP3-33108) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33108) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: YTHD1 Antibody [NBP3-33108] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-YTHD1 antibody (NBP3-33108) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33108) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33108_rabbit-ythd1-pab-29820241435210.jpg)
![Immunocytochemistry/ Immunofluorescence: YTHD1 Antibody [NBP3-33108] - Immunocytochemistry analysis of NIH/3T3 cells labeling YTHD1 with Rabbit anti-YTHD1 antibody (NBP3-33108) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-YTHD1 antibody (NBP3-33108) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33108_rabbit-ythd1-pab-29202410334046.jpg)
![Western Blot: YTHD1 Antibody [NBP2-94472] - Analysis of extracts of various cell lines, using YTHDF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/YTHD1-Antibody-Western-Blot-NBP2-94472-img0001.jpg "Western Blot: YTHD1 Antibody [NBP2-94472] - Analysis of extracts of various cell lines, using YTHDF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: YTHD1 Antibody [NBP1-52865] - Sample Tissue: Human Ovary Tumor Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/YTHD1-Antibody-Western-Blot-NBP1-52865-img0003.jpg "Western Blot: YTHD1 Antibody [NBP1-52865] - Sample Tissue: Human Ovary Tumor Antibody Dilution: 1.0 ug/ml")
![Western Blot: YTHD1 Antibody [NBP1-52865] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: HepG2 cell lysate YTHDF1 is supported by BioGPS gene expression data to be expressed in HepG2](http://images.novusbio.com/fullsize/YTHD1-Antibody-Western-Blot-NBP1-52865-img0004.jpg)
![Western Blot: YTHD1 Antibody [NBP3-09349] - Western blot analysis of YTHD1 in Rat Thymus lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/YTHD1-Antibody-Western-Blot-NBP3-09349-img0001.jpg "Western Blot: YTHD1 Antibody [NBP3-09349] - Western blot analysis of YTHD1 in Rat Thymus lysates. Antibody dilution at 1.0ug/ml")
