FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." alt="HUVECS cells treated with 5 ug/mL Brefeldin A for 5 hours were stained with Human vWF-A1 (RUO grade Caplacizumab Biosimilar) Antibody (Catalog # MAB10591, Filled histogram) or isotype control antibody Llama IgG (open histogram), followed by Goat anti Alpaca Rhodamine Red. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />