STAT1 Antibodies
We offer STAT1 Antibodies for use in common research applications: ELISA, Flow (Intracellular), Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each STAT1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our STAT1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the STAT1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our STAT1 polyclonal antibodies and browse our STAT1 monoclonal antibody catalog.
Alternate Names for STAT1 Antibodies
anti-STAT1 antibody, anti-DKFZp686B04100 antibody, anti-ISGF-3,91kD antibody, anti-signal transducer and activator of transcription 1, 91kDa antibody, anti-signal transducer and activator of transcription-1 antibody
21 Results for "stat1" in Primary Antibodies
![Western Blot: STAT1 [p Ser727] Antibody [NB100-82209] - Analysis of extracts from Hela cells using STAT1(Phospho-Ser727) antibody](http://images.novusbio.com/fullsize/STAT1-[p-Ser727]-Antibody-Western-Blot-NB100-82209-img0001.jpg "Western Blot: STAT1 [p Ser727] Antibody [NB100-82209] - Analysis of extracts from Hela cells using STAT1(Phospho-Ser727) antibody")
![Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody [NB100-82209] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82209 incubated with blocking peptide(right)](http://images.novusbio.com/fullsize/nb100-82209_rabbit-polyclonal-stat1-p-ser727-antibody-103202523464539.jpg)
![Immunocytochemistry/Immunofluorescence: STAT1 [p Ser727] Antibody [NB100-82209] - Staining of methanol-fixed Hela cells using NB100-82209.](http://images.novusbio.com/fullsize/nb100-82209_rabbit-polyclonal-stat1-p-ser727-antibody-113202515145424.jpg)
![Western Blot: STAT1 Antibody (G3-B11-R) [NBP3-32980] - Western blot analysis of STAT1 on different lysates with Mouse anti-STAT1 antibody (NBP3-32980) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: SK-MEL-28 cell lysate Lane 7: MCF7 cell lysate Lane 8: HT-29 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 87/83 kDa Observed band size: 87/83 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32980) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32980_mouse-stat1-mab-g3-b11-r-288202412493435.jpg "Western Blot: STAT1 Antibody (G3-B11-R) [NBP3-32980] - Western blot analysis of STAT1 on different lysates with Mouse anti-STAT1 antibody (NBP3-32980) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: SK-MEL-28 cell lysate Lane 7: MCF7 cell lysate Lane 8: HT-29 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 87/83 kDa Observed band size: 87/83 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32980) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: STAT1 Antibody (G3-B11-R) [NBP3-32980] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-STAT1 antibody (NBP3-32980) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32980) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32980_mouse-stat1-mab-g3-b11-r-29820241492618.jpg)
![Western Blot: STAT1 [p Tyr701] Antibody (PSH04-02) [NBP3-32799] - Western blot analysis of STAT1 on different lysates with Rabbit anti-STAT1 antibody (NBP3-32799) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL IFN for 30 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 50ng/mL IFNa1 for 30 minutes cell lysate Lane 5: A431 cell lysate Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 91/84 kDa (STAT1a/beta ) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32799) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32799_rabbit-stat1-p-tyr701-mab-psh04-02-288202412461612.jpg "Western Blot: STAT1 [p Tyr701] Antibody (PSH04-02) [NBP3-32799] - Western blot analysis of STAT1 on different lysates with Rabbit anti-STAT1 antibody (NBP3-32799) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL IFN for 30 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 50ng/mL IFNa1 for 30 minutes cell lysate Lane 5: A431 cell lysate Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 91/84 kDa (STAT1a/beta ) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32799) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: STAT1 [p Tyr701] Antibody (PSH04-02) [NBP3-32799] - Immunocytochemistry analysis of HeLa cells treated with 100ng/mL IFN alpha 1 for 5 minutes labeling STAT1 with Rabbit anti-STAT1 antibody (NBP3-32799) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-STAT1 antibody (NBP3-32799) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32799_rabbit-stat1-p-tyr701-mab-psh04-02-29202410234276.jpg)
![Western Blot: STAT1 [p Tyr701] Antibody [NBP1-77825] - Shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-a (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.](http://images.novusbio.com/fullsize/STAT1-[p-Tyr701]-Antibody-Western-Blot-NBP1-77825-img0001.jpg "Western Blot: STAT1 [p Tyr701] Antibody [NBP1-77825] - Shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-a (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.")
![Western Blot: STAT1 [p Ser727] Antibody (S09-2G9) [NBP3-20056] - Western blot detection of STAT1 in 3T3 cell lysates using NBP3-20056 (1:1000 diluted). Predicted band size: 87kDa. Observed band size: 87kDa.](http://images.novusbio.com/fullsize/STAT1-p-Ser727-Antibody-S09-2G9-Western-Blot-NBP3-20056-img0001.jpg "Western Blot: STAT1 [p Ser727] Antibody (S09-2G9) [NBP3-20056] - Western blot detection of STAT1 in 3T3 cell lysates using NBP3-20056 (1:1000 diluted). Predicted band size: 87kDa. Observed band size: 87kDa.")
![Immunoprecipitation: STAT1 Antibody [NBP3-14708] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-STAT1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. STAT1 was alsoimmunoprecipitated by rabbit anti-STAT1 antibodyA302-753A. For blotting immunoprecipitated STAT1, was used at 1:1000. Chemiluminescence withan exposure time of 75 seconds.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunoprecipitation-NBP3-14708-img0001.jpg "Immunoprecipitation: STAT1 Antibody [NBP3-14708] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-STAT1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. STAT1 was alsoimmunoprecipitated by rabbit anti-STAT1 antibodyA302-753A. For blotting immunoprecipitated STAT1, was used at 1:1000. Chemiluminescence withan exposure time of 75 seconds.")
![Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.](http://images.novusbio.com/fullsize/p_6190_1.jpg "Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.")
