SPOP Antibodies
We offer SPOP Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each SPOP Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SPOP Antibodies can be used in a variety of model species: Human. Use the list below to choose the SPOP Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SPOP polyclonal antibodies and browse our SPOP monoclonal antibody catalog.
Alternate Names for SPOP Antibodies
anti-SPOP antibody, anti-HIB homolog 1 antibody, anti-Roadkill homolog 1 antibody, anti-speckle-type POZ protein antibody, anti-TEF2 antibody
9 Results for "spop" in Primary Antibodies
![Western Blot: SPOP Antibody [H00008405-B01P] - SPOP inhibits gastric cancer cell proliferation and migration. The expression of SPOP protein in human GC cell lines - MKN45, MKN28, AGS, SGC7901, and human gastric mucosal cell line GES-1 by Western blotting. Image collected and cropped by CiteAb from the following publication (www.jeccr.biomedcentral.com/articles/10.1186/s13046-014-0075-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-H00008405-B01P-img0007.jpg "Western Blot: SPOP Antibody [H00008405-B01P] - SPOP inhibits gastric cancer cell proliferation and migration. The expression of SPOP protein in human GC cell lines - MKN45, MKN28, AGS, SGC7901, and human gastric mucosal cell line GES-1 by Western blotting. Image collected and cropped by CiteAb from the following publication (www.jeccr.biomedcentral.com/articles/10.1186/s13046-014-0075-8) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: SPOP Antibody [H00008405-B01P] - Analysis of purified antibody to SPOP on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/SPOP-Antibody-Immunocytochemistry-Immunofluorescence-H00008405-B01P-img0003.jpg)
![Western Blot: SPOP Antibody [H00008405-B01P] - Analysis of SPOP expression in transfected 293T cell line by SPOP polyclonal antibody. Lane 1: SPOP transfected lysate(41.14 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-H00008405-B01P-img0004.jpg)
![Western Blot: SPOP Antibody [H00008405-B01P] - SPOP expression in gastric cancer. Representative immunoblotting of SPOP protein in gastric cancer samples. Image collected and cropped by CiteAb from the following publication (www.jeccr.biomedcentral.com/articles/10.1186/s13046-014-0075-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-H00008405-B01P-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SPOP Antibody [H00008405-B01P] - SPOP inhibits Hh/Gli signaling activity. SPOP promotes Gli2 degradation through proteasome pathway. MKN45 cells were treated with vehicle (DMSO) or miR-SPOP for 48 h, with or without 10 mM MG-132. In order to limit toxicity, MG-132 was added 4 h before cell harvest. Lysates were subjected to immunoblotting with indicated antibodies. Image collected and cropped by CiteAb from the following publication (www.jeccr.biomedcentral.com/articles/10.1186/s13046-014-0075-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPOP-Antibody-Knockdown-Validated-H00008405-B01P-img0006.jpg)
![Western Blot: SPOP Antibody [H00008405-B01P] - SPOP inhibits gastric cancer cell proliferation & migration. (A) The expression of SPOP protein in human GC cell lines - MKN45, MKN28, AGS, SGC7901, & human gastric mucosal cell line GES-1 by Western blotting. (B) Detection of SPOP expression in transfected AGS cells. Expression of SPOP was detected in cell lysates of SPOP-V5 transfected AGS cells by Western blotting. Control, AGS cells with empty vector plasmid; #1 & #2 represent different groups of stable AGS cell lines transfected with SPOP. (C) Decreased viability of gastric cancer cells transfected with SPOP. The viability of AGS cells was assessed by using an MTT assay. Data represent the average of three experiments (mean ± SD). **P 50 cells. G, histograms show the rate of colony formation. Data represent the average of three experiments (mean ± SD). **P 50 cells. (G) Histograms show the colony formation rate. Data represent the average of three experiments (mean ± SD). **P 50 cells. G, histograms show the rate of colony formation. Data represent the average of three experiments (mean ± SD). **P < 0.01, compared to DMSO treated cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25204354), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008405-b01p_mouse-polyclonal-spop-antibody-310202415485126.jpg)
![Western Blot: SPOP Antibody [H00008405-B01P] - SPOP promotes tumor cell apoptosis. (A) Overexpression of SPOP upregulates the expression of apoptotic proteins. MKN45 cells were transfected with Myc-SPOP & incubated for 48 h. Target proteins in cell lysates were detected by indicated antibodies using Western blotting. (B) Repression of SPOP inhibits cancer cell apoptosis. MKN45 cells were transfected with miR-SPOP-1430 & incubated for 48 h. Target proteins in cell lysates were probed with indicated antibodies using Western blotting. (C) Overexpressed SPOP promotes cleaved Caspase-3 expression. AGS cells were transfected with Myc-SPOP for 48 h & then fixed. Myc-SPOP was detected by incubating cells with mouse anti-Myc antibody & subsequently Alexa Fluor 488 goat anti-mouse antibody. Cleaved Caspase-3 was detected by incubating cells with rabbit anti-cleaved Caspase-3 & subsequently Alexa Fluor 594 donkey anti-rabbit antibody. Nucleus was identified by DAPI staining. (D) The representative photos of cleaved Caspase-3 expression in gastric cancer tissues & adjacent gastric tissues by using immunohistochemical staining (DAB staining, scale bar, 100 μm). Magnified local images reflecting detailed information were shown on the bottom. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25204354), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008405-b01p_mouse-polyclonal-spop-antibody-310202415483629.jpg)
![Western Blot: SPOP Antibody [NBP1-33090] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N1C1] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-NBP1-33090-img0006.jpg "Western Blot: SPOP Antibody [NBP1-33090] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N1C1] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: SPOP Antibody [NBP1-33090] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-NBP1-33090-img0002.jpg)
![Western Blot: SPOP Antibody [NBP1-33090] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N1C1] (NBP1-33090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33090_rabbit-polyclonal-spop-antibody-910202420104467.jpg)
![Western Blot: SPOP Antibody [NBP2-20477] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-NBP2-20477-img0002.jpg "Western Blot: SPOP Antibody [NBP2-20477] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: SPOP Antibody [NBP2-20477] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-NBP2-20477-img0001.jpg)
![Western Blot: SPOP Antibody (JE36-04) [NBP3-32976] - Western blot analysis of SPOP on different lysates with Rabbit anti-SPOP antibody (NBP3-32976) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: LOVO cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32976) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32976_rabbit-spop-mab-je36-04-288202412493437.jpg "Western Blot: SPOP Antibody (JE36-04) [NBP3-32976] - Western blot analysis of SPOP on different lysates with Rabbit anti-SPOP antibody (NBP3-32976) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: LOVO cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32976) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Western Blot: SPOP Antibody [NBP3-35217] - Western blot analysis of various lysates using SPOP Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35217_rabbit-spop-pab-912202415121339.jpg "Western Blot: SPOP Antibody [NBP3-35217] - Western blot analysis of various lysates using SPOP Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: SPOP Antibody (2N8L8) [NBP3-15784] - Western blot analysis of extracts of PC-3 cells, using SPOP Rabbit mAb (NBP3-15784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/SPOP-Antibody-2N8L8-Western-Blot-NBP3-15784-img0001.jpg "Western Blot: SPOP Antibody (2N8L8) [NBP3-15784] - Western blot analysis of extracts of PC-3 cells, using SPOP Rabbit mAb (NBP3-15784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: SPOP Antibody (S01-5B7) [NBP3-19781] - Western blot detection of SPOP in MCF-7 using NBP3-19781. Predicted band size: 42kDa. Observed band size: 42kDa.](http://images.novusbio.com/fullsize/SPOP-Antibody-S01-5B7-Western-Blot-NBP3-19781-img0001.jpg "Western Blot: SPOP Antibody (S01-5B7) [NBP3-19781] - Western blot detection of SPOP in MCF-7 using NBP3-19781. Predicted band size: 42kDa. Observed band size: 42kDa.")
![Western Blot: SPOP Antibody [H00008405-D01P] - Analysis of SPOP expression in transfected 293T cell line by SPOP polyclonal antibody.Lane 1: SPOP transfected lysate(42.10 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPOP-Antibody-Western-Blot-H00008405-D01P-img0002.jpg "Western Blot: SPOP Antibody [H00008405-D01P] - Analysis of SPOP expression in transfected 293T cell line by SPOP polyclonal antibody.Lane 1: SPOP transfected lysate(42.10 KDa).Lane 2: Non-transfected lysate.")
![ELISA: SPOP Antibody (3E2) [H00008405-M04] - Detection limit for recombinant GST tagged SPOP is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SPOP-Antibody-3E2-ELISA-H00008405-M04-img0001.jpg "ELISA: SPOP Antibody (3E2) [H00008405-M04] - Detection limit for recombinant GST tagged SPOP is approximately 0.3ng/ml as a capture antibody.")