RNF146 Antibodies
We offer RNF146 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each RNF146 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RNF146 Antibodies can be used in a variety of model species: Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat. Use the list below to choose the RNF146 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RNF146 polyclonal antibodies.
Alternate Names for RNF146 Antibodies
anti-RNF146 antibody, anti-dactylidin antibody, anti-dJ351K20.1 antibody, anti-DKFZp434O1427 antibody, anti-ring finger protein 146,2610509H23Rik antibody, anti-RP3-351K20.1 antibody
5 Results for "rnf146" in Primary Antibodies
![Western Blot: RNF146 Antibody (PSH0-29) [NBP3-32911] - Western blot analysis of RNF146 on different lysates with Rabbit anti-RNF146 antibody (NBP3-32911) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32911) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32911_rabbit-rnf146-mab-psh0-29-288202412461649.png "Western Blot: RNF146 Antibody (PSH0-29) [NBP3-32911] - Western blot analysis of RNF146 on different lysates with Rabbit anti-RNF146 antibody (NBP3-32911) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32911) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RNF146 Antibody (PSH0-29) [NBP3-32911] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RNF146 antibody (NBP3-32911) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32911) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32911_rabbit-rnf146-mab-psh0-29-298202414304125.jpg)
![Immunocytochemistry/ Immunofluorescence: RNF146 Antibody (PSH0-29) [NBP3-32911] - Immunocytochemistry analysis of NIH/3T3 cells labeling RNF146 with Rabbit anti-RNF146 antibody (NBP3-32911) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RNF146 antibody (NBP3-32911) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32911_rabbit-rnf146-mab-psh0-29-29202410484882.png)
![Flow Cytometry: RNF146 Antibody (PSH0-29) [NBP3-32911] - Flow cytometric analysis of SH-SY5Y cells labeling RNF146. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32911, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black)](http://images.novusbio.com/fullsize/nbp3-32911_rabbit-rnf146-mab-psh0-29-29202416372123.png)
![Western Blot: RNF146 Antibody [NBP3-13191] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RNF146 antibody (NBP3-13191) diluted at 1:1000.](http://images.novusbio.com/fullsize/RNF146-Antibody-Western-Blot-NBP3-13191-img0001.jpg "Western Blot: RNF146 Antibody [NBP3-13191] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RNF146 antibody (NBP3-13191) diluted at 1:1000.")
![Immunohistochemistry-Frozen: RNF146 Antibody [NBP3-13191] - RNF146 antibody detects RNF146 Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: RNF146 stained by RNF146 antibody (NBP3-13191) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/RNF146-Antibody-Immunohistochemistry-Frozen-NBP3-13191-img0002.jpg)
![Western Blot: RNF146 Antibody [H00081847-B01P] - Analysis of RNF146 expression in transfected 293T cell line by RNF146 polyclonal antibody. Lane1:RNF146 transfected lysate(39.38 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/RNF146-Antibody-Western-Blot-H00081847-B01P-img0002.jpg "Western Blot: RNF146 Antibody [H00081847-B01P] - Analysis of RNF146 expression in transfected 293T cell line by RNF146 polyclonal antibody. Lane1:RNF146 transfected lysate(39.38 KDa). Lane2:Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RNF146 Antibody [NBP1-85319] - Staining of human placenta shows moderate cytoplasmic and nuclear positivity in decidual cells.](http://images.novusbio.com/fullsize/RNF146-Antibody-Immunohistochemistry-Paraffin-NBP1-85319-img0004.jpg "Immunohistochemistry-Paraffin: RNF146 Antibody [NBP1-85319] - Staining of human placenta shows moderate cytoplasmic and nuclear positivity in decidual cells.")
![Western Blot: RNF146 Antibody [NBP1-55068] - HepG2 cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/RNF146-Antibody-Western-Blot-NBP1-55068-img0003.jpg "Western Blot: RNF146 Antibody [NBP1-55068] - HepG2 cell lysate, concentration 1.25ug/ml.")