RASEF Antibodies
We offer RASEF Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each RASEF Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RASEF Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the RASEF Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RASEF polyclonal antibodies and browse our RASEF monoclonal antibody catalog.
Alternate Names for RASEF Antibodies
anti-RASEF antibody, anti-FLJ31614 antibody, anti-RAB45 antibody, anti-RAB45, member RAS oncogene family antibody, anti-RAS and EF hand domain containing antibody, anti-RAS and EF-hand domain containing antibody, anti-ras and EF-hand domain-containing protein antibody, anti-Ras-related protein Rab-45 antibody
9 Results for "rasef" in Primary Antibodies
![Western Blot: RASEF Antibody (JE65-33) [NBP3-32886] - Western blot analysis of RASEF on different lysates with Rabbit anti-RASEF antibody (NBP3-32886) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: A431 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 83 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32886) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32886_rabbit-rasef-mab-je65-33-288202412461620.jpg "Western Blot: RASEF Antibody (JE65-33) [NBP3-32886] - Western blot analysis of RASEF on different lysates with Rabbit anti-RASEF antibody (NBP3-32886) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: A431 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 83 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32886) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RASEF Antibody (JE65-33) [NBP3-32886] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-RASEF antibody (NBP3-32886) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32886) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32886_rabbit-rasef-mab-je65-33-298202414304146.jpg)
![Flow Cytometry: RASEF Antibody (JE65-33) [NBP3-32886] - Flow cytometric analysis of Hela cells labeling RASEF. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32886, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32886_rabbit-rasef-mab-je65-33-29202416453269.jpg)
![Western Blot: RASEF Antibody (2D5) [H00158158-M01] - RASEF monoclonal antibody (M01), clone 2D5 Analysis of RASEF expression in K-562.](http://images.novusbio.com/fullsize/RASEF-Antibody-2D5-Western-Blot-H00158158-M01-img0007.jpg "Western Blot: RASEF Antibody (2D5) [H00158158-M01] - RASEF monoclonal antibody (M01), clone 2D5 Analysis of RASEF expression in K-562.")
![Immunohistochemistry-Paraffin: RASEF Antibody (2D5) [H00158158-M01] - Analysis of monoclonal antibody to RASEF on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RASEF-Antibody-2D5-Immunohistochemistry-Paraffin-H00158158-M01-img0005.jpg)
![Sandwich ELISA: RASEF Antibody (2D5) [H00158158-M01] - Detection limit for recombinant GST tagged RASEF is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RASEF-Antibody-2D5-Sandwich-ELISA-H00158158-M01-img0006.jpg)
![Western Blot: RASEF Antibody (S03-1G6) [NBP3-19968] - Western blot detection of RASEF in Jurkat cell lysates using NBP3-19968 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.](http://images.novusbio.com/fullsize/RASEF-Antibody-S03-1G6-Western-Blot-NBP3-19968-img0002.jpg "Western Blot: RASEF Antibody (S03-1G6) [NBP3-19968] - Western blot detection of RASEF in Jurkat cell lysates using NBP3-19968 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.")
![Immunocytochemistry/Immunofluorescence: RASEF Antibody (S03-1G6) [NBP3-19968] - Immunocytochemistry of RASEF (green) in Hela using NBP3-19968 at dilution 1/20, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19968_rabbit-rasef-mab-s03-1g6-113202515145467.jpg)
![Western Blot: RASEF Antibody (1E3) [H00158158-M02] - RASEF monoclonal antibody (M02), clone 1E3. Analysis of RASEF expression in A-431.](http://images.novusbio.com/fullsize/RASEF-Antibody-1E3-Western-Blot-H00158158-M02-img0005.jpg "Western Blot: RASEF Antibody (1E3) [H00158158-M02] - RASEF monoclonal antibody (M02), clone 1E3. Analysis of RASEF expression in A-431.")
![Western Blot: RASEF Antibody (1E3) [H00158158-M02] - RASEF monoclonal antibody (M02), clone 1E3. Analysis of RASEF expression in PC-12.](http://images.novusbio.com/fullsize/RASEF-Antibody-1E3-Western-Blot-H00158158-M02-img0004.jpg)
![Western Blot: RASEF Antibody (1E3) [H00158158-M02] - RASEF monoclonal antibody (M02), clone 1E3. Analysis of RASEF expression in NIH/3T3.](http://images.novusbio.com/fullsize/RASEF-Antibody-1E3-Western-Blot-H00158158-M02-img0006.jpg)
![Western Blot: RASEF Antibody (7L8S8) [NBP3-33294] - Western blot analysis of various lysates, using RASEF Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33294_rabbit-rasef-mab-7l8s8-912202415161898.jpg "Western Blot: RASEF Antibody (7L8S8) [NBP3-33294] - Western blot analysis of various lysates, using RASEF Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: RASEF Antibody (7L8S8) [NBP3-33294] - Western blot analysis of various lysates, using RASEF Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33294_rabbit-rasef-mab-7l8s8-91220241418154.jpg)
![Immunohistochemistry-Paraffin: RASEF Antibody [NBP3-17920] - Staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/RASEF-Antibody-Immunohistochemistry-Paraffin-NBP3-17920-img0001.jpg "Immunohistochemistry-Paraffin: RASEF Antibody [NBP3-17920] - Staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.")
![Western Blot: RASEF Antibody [NBP2-20084] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RASEF-Antibody-Western-Blot-NBP2-20084-img0001.jpg "Western Blot: RASEF Antibody [NBP2-20084] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: RASEF Antibody [NBP2-20083] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RASEF-Antibody-Western-Blot-NBP2-20083-img0001.jpg "Western Blot: RASEF Antibody [NBP2-20083] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: RASEF Antibody [NBP3-09528] - Western blot analysis of RASEF in Fetal Kidney lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/RASEF-Antibody-Western-Blot-NBP3-09528-img0001.jpg "Western Blot: RASEF Antibody [NBP3-09528] - Western blot analysis of RASEF in Fetal Kidney lysates. Antibody dilution at 1.0ug/ml")