| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-88839-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21724-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-88839-50ul | |||
| BSA Free | NBP2-88839-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82169-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21678-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22614-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26514-50ul | |||
![Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-288202412442915.jpg "Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32887) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29820241420957.jpg)
![Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887] - Immunocytochemistry analysis of SH-SY5Y cells labeling Rac1,2,3 with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29202410234241.jpg)
![Western Blot: Antibody (AT2G10) [NBP2-88839] - The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. Jurkat cell lysate](http://images.novusbio.com/fullsize/Antibody-AT2G10-Western-Blot-NBP2-88839-img0002.jpg "Western Blot: Antibody (AT2G10) [NBP2-88839] - The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. Jurkat cell lysate")
![Flow Cytometry: Antibody (AT2G10) [NBP2-88839] - Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained with at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Antibody-AT2G10-Flow-Cytometry-NBP2-88839-img0003.jpg)
![Western Blot: Antibody (AT2G10) [NBP2-88839] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human Rac 1 proteinLane 2.: Recombinant human Rac 2 proteinLane 3.: Recombinant human Rac 3 protein](http://images.novusbio.com/fullsize/Antibody-AT2G10-Western-Blot-NBP2-88839-img0001.jpg)
![Western Blot: Rac1,2,3 Antibody (2J4S6) [NBP3-16547] - Western blot analysis of extracts of various cell lines, using Rac1,2,3 Rabbit mAb (NBP3-16547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Rac1-2-3-Antibody-2J4S6-Western-Blot-NBP3-16547-img0001.jpg "Western Blot: Rac1,2,3 Antibody (2J4S6) [NBP3-16547] - Western blot analysis of extracts of various cell lines, using Rac1,2,3 Rabbit mAb (NBP3-16547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Rac1,2,3 Antibody (SR1332) [NBP3-21724] - Western blot analysis using NBP3-21724 in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21724_rabbit-rac1-2-3-mab-sr1332-6720239533795.jpg "Western Blot: Rac1,2,3 Antibody (SR1332) [NBP3-21724] - Western blot analysis using NBP3-21724 in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.")
![Western Blot: Rac1 Antibody [NB100-82169] - Western blot analysis of extracts from various cells using NB100-82169](http://images.novusbio.com/fullsize/nb100-82169_rabbit-polyclonal-rac1-antibody-23420241452108.jpg "Western Blot: Rac1 Antibody [NB100-82169] - Western blot analysis of extracts from various cells using NB100-82169")
![Immunocytochemistry/ Immunofluorescence: Rac1 Antibody - BSA Free [NB100-82169] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-82169.](http://images.novusbio.com/fullsize/nb100-82169_rabbit-polyclonal-rac1-antibody-103202523464551.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231526123.jpg "Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.")
![Immunocytochemistry/Immunofluorescence: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunofluorescent analysis of MCF-7 cells, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-57202317161873.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Western blot analysis using NBP3-21678 in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231558486.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22614_rabbit-akt1-2-3-mab-sr2003-57202317215156.jpg "Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.")
![Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202410334024.jpg "Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Flow cytometric analysis of MCF7 cells labeling AKT1/2/3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202416453243.jpg)
![Western Blot: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: A549 cell lysateLane 4: U-2 OS cell lysateLane 5: NIH/3T3 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 56 kDaObserved band size: 56 kDaExposure time: 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-3920248453538.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-2722024847194.jpg "Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.")
![Western Blot: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Positive Western Blot detected in: SH-SY5Y whole cell lysate. All lanes: AKT1/2/3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa Observed band size: 56 kDa](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-282202411442636.jpg)
![Flow Cytometry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Overlay Peak curve showing Jurkat cells stained with AKT1/2/3 Antibody (16E10) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-28220241418367.jpg)
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0001.jpg "Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of NIH/3T3 cells, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0002.jpg)