PHLDA1 Antibodies
We offer PHLDA1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each PHLDA1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PHLDA1 Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Rabbit, Rat. Use the list below to choose the PHLDA1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PHLDA1 polyclonal antibodies and browse our PHLDA1 monoclonal antibody catalog.
Alternate Names for PHLDA1 Antibodies
anti-PHLDA1 antibody, anti-Apoptosis-associated nuclear protein antibody, anti-DT1P1B11 antibody, anti-MGC131738 antibody, anti-PHRIPT-cell death-associated gene 51 protein antibody, anti-pleckstrin homology-like domain family A member 1 antibody, anti-pleckstrin homology-like domain, family A, member 1 antibody, anti-PQ-rich protein antibody, anti-Proline- and glutamine-rich protein antibody, anti-Proline- and histidine-rich protein antibody, anti-proline-histidine rich protein antibody, anti-TDAG51PQR protein antibody
6 Results for "phlda1" in Primary Antibodies
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Analysis in human salivary gland and skeletal muscle tissues using NBP1-84969 antibody. Corresponding PHLDA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-29620238107..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Analysis in human salivary gland and skeletal muscle tissues using NBP1-84969 antibody. Corresponding PHLDA1 RNA-seq data are presented for the same tissues.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: PHLDA1 Antibody [NBP1-84969] - PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock and indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-Western-Blot-NBP1-84969-img0007.jpg)
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] - Staining of human salivary gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84969-img0004.jpg)
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-296202381239..jpg)
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-29620238148..jpg)
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-296202381536..jpg)
![Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-84969_rabbit-polyclonal-phlda1-antibody-immunohistochemistry-paraffin-296202381650..jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse and subjected to heat shock after 2, 4, and 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids and condensing spermatids are marked with arrowhead and arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-2092023181151.jpg)
![Immunocytochemistry/ Immunofluorescence: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. (a) Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse & subjected to heat shock after 2, 4, & 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids & condensing spermatids are marked with arrowhead & arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. (b) Detection of apoptotic DNA breaks (by TUNEL assay, red; DNA stained with DAPI, blue) in seminiferous tubules of untreated mice & after heat shock in vivo & indicated recovery time (2–6 h). (c) Detection of PHLDA1 by immunofluorescence (red; DNA stained with DAPI, blue) in cryptorchid testes 7 & 14 days after surgery (upper panel) & detection of apoptotic DNA breaks (by TUNEL assay, red) in corresponding tissues (bottom panel). Scale bar—50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-310202416114768.jpg)
![Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-310202416114735.jpg)
![Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84969_rabbit-polyclonal-phlda1-antibody-310202416114722.jpg)
![Immunocytochemistry/Immunofluorescence: PHLDA1 Antibody (3A5) [H00022822-M08] - Analysis of monoclonal antibody to PHLDA1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PHLDA1-Antibody-3A5-Immunocytochemistry-Immunofluorescence-H00022822-M08-img0001.jpg "Immunocytochemistry/Immunofluorescence: PHLDA1 Antibody (3A5) [H00022822-M08] - Analysis of monoclonal antibody to PHLDA1 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: PHLDA1 Antibody (3A5) [H00022822-M08] - Detection limit for recombinant GST tagged PHLDA1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-3A5-Sandwich-ELISA-H00022822-M08-img0002.jpg)
![Western Blot: PHLDA1 Antibody - BSA Free [NBP3-47158] - HeLa cells were subjected to SDS PAGE followed by western blot with (PHLDA1 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-47158_rabbit-phlda1-pab-bsa-free-284202512275018.jpg "Western Blot: PHLDA1 Antibody - BSA Free [NBP3-47158] - HeLa cells were subjected to SDS PAGE followed by western blot with (PHLDA1 antibody) at dilution of 1:500")
![Immunocytochemistry/Immunofluorescence: PHLDA1 Antibody [NBP2-56404] - Staining of human cell line PC-3 shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56404-img0001.jpg "Immunocytochemistry/Immunofluorescence: PHLDA1 Antibody [NBP2-56404] - Staining of human cell line PC-3 shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Western Blot: PHLDA1 Antibody [NBP1-53066] - Human Placenta lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-Western-Blot-NBP1-53066-img0002.jpg "Western Blot: PHLDA1 Antibody [NBP1-53066] - Human Placenta lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PHLDA1 Antibody [NBP1-53065] - Human Placenta lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PHLDA1-Antibody-Western-Blot-NBP1-53065-img0002.jpg "Western Blot: PHLDA1 Antibody [NBP1-53065] - Human Placenta lysate, concentration 0.2-1 ug/ml.")