OTUD6A Antibodies
We offer OTUD6A Antibodies for use in common research applications: Western Blot. Each OTUD6A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our OTUD6A Antibodies can be used in a variety of model species: Human. Use the list below to choose the OTUD6A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our OTUD6A polyclonal antibodies.
Alternate Names for OTUD6A Antibodies
anti-OTUD6A antibody, anti-DUBA-2 antibody, anti-DUBA2 antibody, anti-OTU domain containing 6A antibody
5 Results for "otud6a" in Primary Antibodies
![Western Blot: OTUD6A Antibody [NBP1-91498] - Titration: 2 ug/ml Positive Control: Human HeLa Cell line.](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Western-Blot-NBP1-91498-img0003.jpg "Western Blot: OTUD6A Antibody [NBP1-91498] - Titration: 2 ug/ml Positive Control: Human HeLa Cell line.")
![Western Blot: OTUD6A Antibody [NBP1-91498] - Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate.](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Western-Blot-NBP1-91498-img0002.jpg)
![Western Blot: OTUD6A Antibody [NBP1-91498] - OTUD6A deubiquitinates & affects Drp1 stability. Cells were infected with pLKO‐shOTUD6A or mock virus, selected by puromycin for 3 days to eliminate noninfected cells. (A) Depletion of OTUD6A in cells leads to decreased Drp1. Relative OTUD6A (B) & Drp1 (C) expression of (A) was further quantified. (D) Exogenous expression of OTUD6A causes the accumulation of Drp1 in HCT116 (left) & DLD1 (right) cells. (E) Relative Drp1 expression of (D) was further quantified. (F) HCT116 cells transfected with pLKO‐shOTUD6A were left untreated or treated with proteasome inhibitor MG132 (10 μm, 12 h), & the proteins were extracted & subjected to western blotting. (G) Relative Drp1 expression of (F) was further quantified. (H, I) Half‐life analysis of Drp1 in HCT116 cells with & without knockdown of OTUD6A. (J, K) Half‐life analysis of Drp1 in DLD1 cells expressing OTUD6A. (L) OTUD6A inhibits Drp1 ubiquitination in cells. IB analysis of Ni‐NTA pull‐down products & WCL derived from HEK293 cells transfected with the indicated constructs. (M) OTUD6A WT but not the mutant C152A deubiquitinates Drp1. The mean ± SD for three independent experiments is shown. For two groups, data were analyzed by unpaired, two‐tailed Student's t‐test. **P < 0.01, ***P < 0.001. Other data were analyzed by one‐way ANOVA, followed by a Bonferroni post hoc test. **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33070427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91498_rabbit-polyclonal-otud6a-antibody-31020241611478.jpg)
![Western Blot: OTUD6A Antibody [NBP1-91498] - OTUD6A deubiquitinates & affects Drp1 stability. Cells were infected with pLKO‐shOTUD6A or mock virus, selected by puromycin for 3 days to eliminate noninfected cells. (A) Depletion of OTUD6A in cells leads to decreased Drp1. Relative OTUD6A (B) & Drp1 (C) expression of (A) was further quantified. (D) Exogenous expression of OTUD6A causes the accumulation of Drp1 in HCT116 (left) & DLD1 (right) cells. (E) Relative Drp1 expression of (D) was further quantified. (F) HCT116 cells transfected with pLKO‐shOTUD6A were left untreated or treated with proteasome inhibitor MG132 (10 μm, 12 h), & the proteins were extracted & subjected to western blotting. (G) Relative Drp1 expression of (F) was further quantified. (H, I) Half‐life analysis of Drp1 in HCT116 cells with & without knockdown of OTUD6A. (J, K) Half‐life analysis of Drp1 in DLD1 cells expressing OTUD6A. (L) OTUD6A inhibits Drp1 ubiquitination in cells. IB analysis of Ni‐NTA pull‐down products & WCL derived from HEK293 cells transfected with the indicated constructs. (M) OTUD6A WT but not the mutant C152A deubiquitinates Drp1. The mean ± SD for three independent experiments is shown. For two groups, data were analyzed by unpaired, two‐tailed Student's t‐test. **P < 0.01, ***P < 0.001. Other data were analyzed by one‐way ANOVA, followed by a Bonferroni post hoc test. **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33070427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91498_rabbit-polyclonal-otud6a-antibody-310202416121998.jpg)
![Western Blot: OTUD6A Antibody [NBP1-91498] - Decreased cancer cell growth induced by OTUD6A can be partly restored by Drp1 expression. (A) HeLa cells were infected with pLKO‐shOTUD6A, selected by puromycin for 3 days. OTUD6A−/− cells were then infected with Drp1‐WT or Drp1‐ delta MDVD, selected by hygro for 3 days. (B) Fluorescence staining of mitochondrial morphology in HeLa cells stably knockdown OTUD6A & express Drp1‐WT or Drp1‐ delta MDVD. The magnification of the images is 400×. (C) The length of mitochondrial fragments in the indicated HeLa cells. (D) Cell growth of indicated cells. (E) Colony formation of the indicated cells. (F) Relative colony numbers were further quantified to determine the extent of the colony formation. The mean ± SD for three independent experiments is shown. Data were analyzed by one‐way or two‐way ANOVA, followed by a Bonferroni post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001. For image C & F, *P < 0.05, ***P < 0.001 GFP vs WT. #P < 0.05, ###P < 0.001 WT vs. delta MDVD; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001 shGFP + GFP vs. shOTUD6A. For image D, ***P < 0.001 shGFP + GFP vs. shOTUD6A + GFP; ^^^P < 0.001 shGFP + GFP vs. shOTUD6A + delta MDVD. Scale bar represents 40 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33070427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91498_rabbit-polyclonal-otud6a-antibody-31020241612225.jpg)
![Western Blot: OTUD6A Antibody [NBP1-91498] - OTUD6A interacts with Drp1. (A) Drp1 specifically interacts with OTUD6A in cells. IB analysis of IP & WCL derived from HEK293 cells transfected with HA‐Drp1 & the indicated Flag‐DUBs for 48 h. (B) Overview of OTUD6A & Drp1 structures. (C) OTUD6A WT but not mutant C152A specifically interacts with Drp1. (D) HEK293 cells transfected with the Drp1 constructs were subjected to IP with the anti‐HA or anti‐Flag antibodies. (E) Drp1 interacts with endogenous OTUD6A in cells. HEK293 cell lysates were subjected to pull down by anti‐Drp1 antibody & protein A/G agarose, followed by IB analysis with the indicated antibodies. (F) HeLa cells were fixed & immunostained with anti‐Drp1 & anti‐OTUD6A before confocal microscopy. The magnification of the images is 400×. (G) Quantification of the colocalization between Drp1 & OTUD6A. Scale bar represents 40 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33070427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91498_rabbit-polyclonal-otud6a-antibody-3102024161222.jpg)
![Western Blot: OTUD6A Antibody [NBP1-91498] - OTUD6A deubiquitinates & affects Drp1 stability. Cells were infected with pLKO‐shOTUD6A or mock virus, selected by puromycin for 3 days to eliminate noninfected cells. (A) Depletion of OTUD6A in cells leads to decreased Drp1. Relative OTUD6A (B) & Drp1 (C) expression of (A) was further quantified. (D) Exogenous expression of OTUD6A causes the accumulation of Drp1 in HCT116 (left) & DLD1 (right) cells. (E) Relative Drp1 expression of (D) was further quantified. (F) HCT116 cells transfected with pLKO‐shOTUD6A were left untreated or treated with proteasome inhibitor MG132 (10 μm, 12 h), & the proteins were extracted & subjected to western blotting. (G) Relative Drp1 expression of (F) was further quantified. (H, I) Half‐life analysis of Drp1 in HCT116 cells with & without knockdown of OTUD6A. (J, K) Half‐life analysis of Drp1 in DLD1 cells expressing OTUD6A. (L) OTUD6A inhibits Drp1 ubiquitination in cells. IB analysis of Ni‐NTA pull‐down products & WCL derived from HEK293 cells transfected with the indicated constructs. (M) OTUD6A WT but not the mutant C152A deubiquitinates Drp1. The mean ± SD for three independent experiments is shown. For two groups, data were analyzed by unpaired, two‐tailed Student's t‐test. **P < 0.01, ***P < 0.001. Other data were analyzed by one‐way ANOVA, followed by a Bonferroni post hoc test. **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33070427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91498_rabbit-polyclonal-otud6a-antibody-31020241613625.jpg)
![Western Blot: OTUD6A Antibody [NBP1-91498] - OTUD6A deubiquitinates & affects Drp1 stability. Cells were infected with pLKO‐shOTUD6A or mock virus, selected by puromycin for 3 days to eliminate noninfected cells. (A) Depletion of OTUD6A in cells leads to decreased Drp1. Relative OTUD6A (B) & Drp1 (C) expression of (A) was further quantified. (D) Exogenous expression of OTUD6A causes the accumulation of Drp1 in HCT116 (left) & DLD1 (right) cells. (E) Relative Drp1 expression of (D) was further quantified. (F) HCT116 cells transfected with pLKO‐shOTUD6A were left untreated or treated with proteasome inhibitor MG132 (10 μm, 12 h), & the proteins were extracted & subjected to western blotting. (G) Relative Drp1 expression of (F) was further quantified. (H, I) Half‐life analysis of Drp1 in HCT116 cells with & without knockdown of OTUD6A. (J, K) Half‐life analysis of Drp1 in DLD1 cells expressing OTUD6A. (L) OTUD6A inhibits Drp1 ubiquitination in cells. IB analysis of Ni‐NTA pull‐down products & WCL derived from HEK293 cells transfected with the indicated constructs. (M) OTUD6A WT but not the mutant C152A deubiquitinates Drp1. The mean ± SD for three independent experiments is shown. For two groups, data were analyzed by unpaired, two‐tailed Student's t‐test. **P < 0.01, ***P < 0.001. Other data were analyzed by one‐way ANOVA, followed by a Bonferroni post hoc test. **P < 0.01, ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33070427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91498_rabbit-polyclonal-otud6a-antibody-310202416121984.jpg)
![Immunohistochemistry-Paraffin: OTUD6A Antibody [NBP2-49078] - Staining in human testis and endometrium tissues using anti-OTUD6A antibody. Corresponding OTUD6A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Immunohistochemistry-Paraffin-NBP2-49078-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OTUD6A Antibody [NBP2-49078] - Staining in human testis and endometrium tissues using anti-OTUD6A antibody. Corresponding OTUD6A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: OTUD6A Antibody [NBP2-49078] - Analysis in control (vector only transfected HEK293T lysate) and OTUD6A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Western-Blot-NBP2-49078-img0006.jpg)
![Immunohistochemistry-Paraffin: OTUD6A Antibody [NBP2-49078] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Immunohistochemistry-Paraffin-NBP2-49078-img0003.jpg)
![Immunohistochemistry-Paraffin: OTUD6A Antibody [NBP2-49078] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Immunohistochemistry-Paraffin-NBP2-49078-img0004.jpg)
![Western Blot: OTUD6A Antibody [NBP1-91497] - Human Hela cell line, 15 micrograms per LaneUsed at both 1 : 500 and 1 : 1000 (in 5% Milk in PBS/0.05% Tween). Primary was incubated for 2hrs at 4 degrees Celsius. Both work, but 1 : 500 gave a stronger signal.](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Western-Blot-NBP1-91497-img0002.jpg "Western Blot: OTUD6A Antibody [NBP1-91497] - Human Hela cell line, 15 micrograms per LaneUsed at both 1 : 500 and 1 : 1000 (in 5% Milk in PBS/0.05% Tween). Primary was incubated for 2hrs at 4 degrees Celsius. Both work, but 1 : 500 gave a stronger signal.")
![Western Blot: OTUD6A Antibody [NBP1-91497] - Titration: 0.2-1 ug/ml, Positive Control: MCF7 cell lysate](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Western-Blot-NBP1-91497-img0001.jpg)
![Western Blot: OTUD6A Antibody [NBP2-94731] - Analysis of extracts of various cell lines, using OTUD6A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/OTUD6A-Antibody-Western-Blot-NBP2-94731-img0001.jpg "Western Blot: OTUD6A Antibody [NBP2-94731] - Analysis of extracts of various cell lines, using OTUD6A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: OTUD6A Antibody - BSA Free [NBP3-47243] - MCF-7 cells were subjected to SDS PAGE followed by western blot with ( OTUD6A Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47243_rabbit-otud6a-pab-bsa-free-28420251235662.jpg "Western Blot: OTUD6A Antibody - BSA Free [NBP3-47243] - MCF-7 cells were subjected to SDS PAGE followed by western blot with ( OTUD6A Antibody) at dilution of 1:1000")