Mesothelin Antibodies
We offer Mesothelin Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each Mesothelin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Mesothelin Antibodies can be used in a variety of model species: Human, Primate. Use the list below to choose the Mesothelin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Mesothelin polyclonal antibodies and browse our Mesothelin monoclonal antibody catalog.
Alternate Names for Mesothelin Antibodies
anti-Mesothelin antibody, anti-MSLN antibody, anti-CAK1 antibody, anti-CAK1 antigen antibody, anti-megakaryocyte potentiating factor antibody, anti-MPFSMRP antibody, anti-Pre-pro-megakaryocyte-potentiating factor antibody, anti-soluble MPF mesothelin related protein antibody
42 Results for "mesothelin" in Primary Antibodies
![Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Biotin-conjugated Mesothelin Mouse Monoclonal Antibody (MSLN/2131).](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MSLN-2131-Immunohistochemistry-Paraffin-NBP2-79724-img0008.jpg "Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Biotin-conjugated Mesothelin Mouse Monoclonal Antibody (MSLN/2131).")
![Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Formalin-fixed, paraffin-embedded human Lung Mesothelioma stained with Mesothelin Antibody (MSLN/2131).](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MSLN-2131-Immunohistochemistry-Paraffin-NBP2-79724-img0001.jpg)
![Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Mesothelin Antibody (MSLN/2131).](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MSLN-2131-Immunohistochemistry-Paraffin-NBP2-79724-img0002.jpg)
![Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Formalin-fixed, paraffin-embedded human Tonsil stained with Mesothelin Antibody (MSLN/2131).](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MSLN-2131-Immunohistochemistry-Paraffin-NBP2-79724-img0003.jpg)
![Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Formalin-fixed, paraffin-embedded Mouse Lung stained with Mesothelin Antibody (MSLN/2131).](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MSLN-2131-Immunohistochemistry-Paraffin-NBP2-79724-img0004.jpg)
![Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Formalin-fixed, paraffin-embedded Rat Stomach stained with Mesothelin Antibody (MSLN/2131).](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MSLN-2131-Immunohistochemistry-Paraffin-NBP2-79724-img0005.jpg)
![Protein Array: Mesothelin Antibody (MSLN/2131) [NBP2-79724] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Mesothelin Antibody (MSLN/2131). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MSLN-2131-Protein-Array-NBP2-79724-img0006.jpg)
![Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Western blotting using Mesothelin antibody (MB-G10). Load: Mesothelin-Fc (lane 1, 100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng)Primary antibody: anti-Mesothelin at 1mg/ml. Secondary Antibody: ALP goat anti-mouse IgG and BCIP/NBT substrate.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MB-G10-Western-Blot-NB110-85538-img0008.jpg "Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Western blotting using Mesothelin antibody (MB-G10). Load: Mesothelin-Fc (lane 1, 100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng)Primary antibody: anti-Mesothelin at 1mg/ml. Secondary Antibody: ALP goat anti-mouse IgG and BCIP/NBT substrate.")
![Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538] - Immunohistochemistry using Mesothelin antibody (MB-G10) to react with two epitopes on Mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-Mesothelin primary antibodies were used at 10 ug/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to Equineradish peroxidase at 25 ug/mL in 1% BSA/PBS for 30 minutes. (magnification, x200; bar, 50 um). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MB-G10-Immunohistochemistry-NB110-85538-img0010.jpg)
![SDS-Page: Mesothelin Antibody (MB-G10) [NB110-85538] - SDS-PAGE of Mesothelin antibody (MB-G10). Lane 1: Non-Reduced Mesothelin antibody (MB-G10). Lane M: 3 uL OPAL Pre-stained Marker. Lane 2: Reduced Mesothelin antibody (MB-G10). Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 160 kDa; Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MB-G10-SDS-Page-NB110-85538-img0009.jpg)
![Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-88224] - Staining in human fallopian tube and lymph node tissues using anti-MSLN antibody. Corresponding MSLN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-Immunohistochemistry-Paraffin-NBP1-88224-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-88224] - Staining in human fallopian tube and lymph node tissues using anti-MSLN antibody. Corresponding MSLN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Mesothelin Antibody [NBP1-88224] - Analysis in human cell lines HeLa and U-251MG using anti-MSLN antibody. Corresponding MSLN RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-Western-Blot-NBP1-88224-img0010.jpg)
![Western Blot: Mesothelin Antibody [NBP1-88224] - Analysis in control (vector only transfected HEK293T lysate) and MSLN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Mesothelin-Antibody-Western-Blot-NBP1-88224-img0011.jpg)
![Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-88224] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-Immunohistochemistry-Paraffin-NBP1-88224-img0001.jpg)
![Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-88224] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-Immunohistochemistry-Paraffin-NBP1-88224-img0008.jpg)
![Western Blot: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: NCI-H226 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lane 5: PC-3M cell lysate (negative) Lane 6: A549 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 40 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32593) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-27820241529554.jpg "Western Blot: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: NCI-H226 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lane 5: PC-3M cell lysate (negative) Lane 6: A549 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 40 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32593) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunofluorescence analysis of paraffin-embedded human ovarian carcinoma tissue labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32593, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-29820241492656.jpg)
![Immunohistochemistry: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32593) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-298202414161861.jpg)
![Immunocytochemistry/ Immunofluorescence: Mesothelin Antibody (PSH02-30) [NBP3-32593] - Immunocytochemistry analysis of NCI-H226 cells labeling Mesothelin with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mesothelin antibody (NBP3-32593) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32593_rabbit-mesothelin-mab-psh02-30-2920241192384.jpg)