MAG/Siglec-4a Antibodies

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to processes of oligodendrocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to processes of oligodendrocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="MAG/Siglec‑4a was detected in perfusion fixed frozen sections of rat brain (cerebellum) using 25 µg/mL Goat Anti-Rat MAG/Siglec‑4a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF538) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to processes of oligodendrocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for MAG/Siglec‑4a at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for MAG/Siglec‑4a at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of human cerebellum, mouse cerebellum and rat cerebellum. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human MAG/Siglec‑4a Monoclonal Antibody (Catalog # MAB11688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MAG/Siglec‑4a at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF018). A specific band was detected for MAG/Siglec‑4a at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for MAG/Siglec‑4a at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of human cerebellum, mouse cerebellum and rat cerebellum. PVDF membrane was probed with 0.1 µg/ml of Mouse Anti-Human MAG/Siglec‑4a Monoclonal Antibody (Catalog # MAB11687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MAG/Siglec‑4a at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human MAG/Siglec‑4a Monoclonal Antibody (Catalog # MAB11687) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the mouse hippocampus." class="big_lightbox"> 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human MAG/Siglec‑4a Monoclonal Antibody (Catalog # MAB11687) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the mouse hippocampus." class="big_thumb" />

538-MG), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat MAG/Siglec-4a Fc Chimera (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat MAG/Siglec-4a Monoclonal Antibody (Catalog # MAB538). At 40 µg/mL, this antibody will neutralize approximately 60-85% of Recombinant Rat MAG/Siglec-4a-dependent adhesion of human red blood cells." class="big_lightbox"> 538-MG), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat MAG/Siglec-4a Fc Chimera (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat MAG/Siglec-4a Monoclonal Antibody (Catalog # MAB538). At 40 µg/mL, this antibody will neutralize approximately 60-85% of Recombinant Rat MAG/Siglec-4a-dependent adhesion of human red blood cells." alt="Recombinant Rat MAG/Siglec-4a Fc Chimera (Catalog # 538-MG), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat MAG/Siglec-4a Fc Chimera (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat MAG/Siglec-4a Monoclonal Antibody (Catalog # MAB538). At 40 µg/mL, this antibody will neutralize approximately 60-85% of Recombinant Rat MAG/Siglec-4a-dependent adhesion of human red blood cells." class="big_thumb" />