LAG-3 Antibodies

HAF018). A specific band was detected for LAG-3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for LAG-3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human LAG-3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LAG-3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_lightbox"> MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." alt="Mouse splenocytes were treated for 72 hours with 50 ng/mL PMA and 500 ng/mL Ca²⁺Ionomycin then stained with Rat Anti-Mouse LAG-3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_thumb" />

MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="LAG‑3 was detected in immersion fixed paraffin-embedded sections of tonsil using Mouse Anti-Human LAG‑3 Monoclonal Antibody (Catalog # MAB231971) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were either untreated (bottom panel) or treated with with 5 µg/mL PHA (top panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23194) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />