IL-27 p28/IL-30 Antibodies

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="IL-27 p28/IL-30 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using 15 µg/mL Mouse IL-27 p28/ IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL-27 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-27 p28/IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL-27 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-27 p28/IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

HAF005). A specific band was detected for IL-27 p28/IL-30 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for IL-27 p28/IL-30 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CHO Chinese hamster ovary cell line transfected with mouse IL-27 p28/IL-30. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-27 p28/IL-30 Monoclonal Antibody (Catalog # MAB7430) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-27 p28/IL-30 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB0061, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB0061, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="RAW 264.7 mouse monocyte/macrophage cell line was unstimulated (light orange open histogram) or treated with 1 µg/mL LPS for 20 hours, then stained with Rat Anti-Mouse IL-27 p28/IL-30 Monoclonal Antibody (Catalog # MAB1834, dark orange filled histogram) or isotype control (Catalog # MAB0061, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />