IL-27 p28/IL-30 Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-27 p28/IL-30 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-27 p28/IL-30
  Phe29-Ser234
  Accession # Q8K3I6
• Specificity: Detects mouse IL-27 p28/IL-30 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Il27
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV‑induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2‑4). The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and a 210 aa mature protein (5). The mature region contains two potential N‑linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues that place the molecule in the type I cytokine receptor family (5). As with p40, (the EBI3 counterpart in IL‑12), IL‑27 EBI3 is reported to form homodimers (6). Mouse EBI3 is 61% and 66% aa identical to human and bovine EBI3. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and a 206 aa mature region (7). The mature region is characterized by the presence of one potential N‑linked glycosylation site and four alpha ‑helices, placing it in the IL‑6 family of helical cytokines. Mouse p28 is 74% aa identical to human p28. IL‑27 is expressed by monocytes, endothelial cells and dendritic cells (8). IL‑27 binds to and signals through a heterodimeric receptor complex composed of WSX‑1 (TCCR) and gp130. Evidence suggests IL‑27 interacts only with WSX‑1 (7, 9, 10). IL‑27 has both anti-  and proinflammatory properties. As an anti‑inflammatory, IL‑27 seems to induce a general negative feedback program that limits T  and NK-T cell activity (3, 8). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4⁺ T cells, making them susceptible to subsequent IL-12 activit  (and possible Th1 development) (6). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL‑27 secretion through TLR4, and also block IL-27 production via C5a induction (11).

1. Boulay, J-L. et al. (2003) Immunity 19:159.
2. Trinchieri, G. et al. (2003) Immunity 19:641.
3. Murakami, M. et al. (2004) Growth Factors 22:75.
4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Expert Opin. Biol. Ther. 3:715.
5. Nomura, H. et al. (1997) GenBank Accession # O35228.
6. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl). 193:1.
7. Pflanz, S. et al. (2002) Immunity 16:779.
8. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
9. Pflanz, S. et al. (2004) J. Immunol. 172: 2225.
10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
11. Hawlish, H. et al. (2005) Immunity 22:415.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Il27