IFN-gamma Antibodies

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IFN-gamma was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Bovine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2300) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

2300-BG) was serially diluted and captured by Rat Anti-Bovine IFN‑ gamma Monoclonal Antibody (Catalog # MAB23001) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2300) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2300-BG) was serially diluted and captured by Rat Anti-Bovine IFN‑ gamma Monoclonal Antibody (Catalog # MAB23001) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2300) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985). The ND₅₀ is typically 0.6-3.0 µg/mL." class="big_lightbox"> 985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985). The ND₅₀ is typically 0.6-3.0 µg/mL." alt="Recombinant Porcine IFN-gamma (985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985). The ND₅₀ is typically 0.6-3.0 µg/mL." class="big_thumb" />

MAB9852) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB9852) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated or untreated with calcium ionomycin and PMA using Rabbit Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB2853) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN-gamma Monoclonal Antibody (Catalog # MAB285R). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_lightbox"> 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN-gamma Monoclonal Antibody (Catalog # MAB285R). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_thumb" />

2300-BG) was serially diluted and captured by Rat Anti-Bovine IFN‑ gamma Monoclonal Antibody (Catalog # MAB23001) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2300) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2300-BG) was serially diluted and captured by Rat Anti-Bovine IFN‑ gamma Monoclonal Antibody (Catalog # MAB23001) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2300) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Bovine IFN‑ gamma (Catalog # 2300-BG) was serially diluted and captured by Rat Anti-Bovine IFN‑ gamma Monoclonal Antibody (Catalog # MAB23001) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2300) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

985-PI) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IFN-gamma Monoclonal Antibody (Catalog # MAB9851). The ND₅₀ is typically 15-60 ng/mL." class="big_lightbox"> 985-PI) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IFN-gamma Monoclonal Antibody (Catalog # MAB9851). The ND₅₀ is typically 15-60 ng/mL." alt="Recombinant Porcine IFN-gamma (Catalog # 985-PI) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IFN-gamma Monoclonal Antibody (Catalog # MAB9851). The ND₅₀ is typically 15-60 ng/mL." class="big_thumb" />

DY990). Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Porcine IFN-gamma protein was serially diluted 2-fold and captured by Mouse Anti-Porcine IFN-gamma Monoclonal Antibody (Catalog # MAB9852) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_lightbox"> F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " alt="Human peripheral blood mononuclear cells (PBMCs) treated with (A) 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight or (B) untreated, were stained with Mouse Anti-Human IFN- gamma Monoclonal Antibody (Catalog # MAB285R-FAB2) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_thumb" />

985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9851). The ND₅₀ is typically 1-6 ng/mL." class="big_lightbox"> 985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9851). The ND₅₀ is typically 1-6 ng/mL." alt="Recombinant Porcine IFN-gamma (Catalog # 985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN-gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9851). The ND₅₀ is typically 1-6 ng/mL." class="big_thumb" />