Human IgM Antibodies
We offer Human IgM Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Radioimmunoassay, Western Blot. Each Human IgM Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Human IgM Antibodies can be used in a variety of model species: Human. Use the list below to choose the Human IgM Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Human IgM monoclonal antibodies.
Alternate Names for Human IgM Antibodies
anti-Human IgM antibody
3 Results for "human-igm" in Primary Antibodies
![Western Blot: Human IgM Antibody (P5E2) [NBP1-78603] - Analysis of 1ug of purified Human IgM, Human IgA, and Human IgG and 10ul of PageRuler Prestained Protein Ladder per well.](http://images.novusbio.com/fullsize/Human-IgM-Antibody-P5E2-Western-Blot-NBP1-78603-img0004.jpg "Western Blot: Human IgM Antibody (P5E2) [NBP1-78603] - Analysis of 1ug of purified Human IgM, Human IgA, and Human IgG and 10ul of PageRuler Prestained Protein Ladder per well.")
![Flow Cytometry: Human IgM Antibody (P5E2) [NBP1-78603] - Analysis of surface IgM expression was performed on Ramos (surface and cytoplasmic IgG/lambda-expressing B cells), Raji (cytoplasmic IgG/kappa-expressing B cells), and Jurkat (T cells) cell lines. Cells were stained with a mouse anti-human IgM monoclonal antibody or mouse IgG1 isotype control (black histogram), each at a concentration of 1ug/ml. After incubation of the primary antibody on ice for 1 hour, the cells were stained with a FITC-conjugated goat anti-mouse IgG Fc secondary antibody at a dilution of 1:500 for 30 minutes on ice. The representative 10,000 cells were obtained for each sample.](http://images.novusbio.com/fullsize/Human-IgM-Antibody-P5E2-Flow-Cytometry-NBP1-78603-img0003.jpg)
![ELISA: Human IgM Antibody (P5E2) [NBP1-78603] - Direct ELISA analysis of various immunoglobulin (Ig) isotypes was performed by coating wells of a 96-well plate with 100ul per well of Human IgM, Human IgG, or Human IgA diluted in carbonate/bicarbonate buffer, starting at a concentration of 50ng/ml and serially diluting 2-fold to a concentration of 0.05ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse anti-human IgM monoclonal antibody at a concentration of 1ug/ml for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG Fc secondary antibody at a dilution of 1:8000 for 1 hour at room temperature. Detection was performed using 1-Step Ultra TMB substrate for 5 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.](http://images.novusbio.com/fullsize/Human-IgM-Antibody-P5E2-ELISA-NBP1-78603-img0001.jpg)
![ELISA: Human IgM Antibody (P5E2) [NBP1-78603] - Direct ELISA analysis of IgM was performed by coating wells of a 96-well plate with 100ul per well of Human IgM diluted in carbonate/bicarbonate buffer, starting at a concentration of 5ug/ml and serially diluting 2-fold to a concentration of 5ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse anti-human IgM monoclonal antibody at a concentration of 1ug/ml for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG Fc secondary antibody at a dilution of 1:8000 for 1 hour at room temperature. Detection was performed using 1-Step Ultra TMB substrate for 5 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.](http://images.novusbio.com/fullsize/Human-IgM-Antibody-P5E2-ELISA-NBP1-78603-img0002.jpg)