GFP Antibodies

HAF018). A specific band was detected for GFP at approximately 28 and recombinant RANGAP-1 tagged with eYFP at 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for GFP at approximately 28 and recombinant RANGAP-1 tagged with eYFP at 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows synthetic eGFP, recombinant RANGAP-1 tagged with eYFP, recombinant GRPuv, and crystal jelly GFP. PVDF membrane was probed with 2 µg/mL of Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42402) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GFP at approximately 28 and recombinant RANGAP-1 tagged with eYFP at 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB0041, data not shown) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_lightbox"> MAB0041, data not shown) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_thumb" />

NL007). Lower panel shows transfected cells by GFPuv auto-fluorescence (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007). Lower panel shows transfected cells by GFPuv auto-fluorescence (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="GFP was detected in 2% paraformaldehyde immersion fixed HEK293 human embryonic kidney cell line transfected with GFP-expressing protein using Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007). Lower panel shows transfected cells by GFPuv auto-fluorescence (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="HEK293 human embryonic kidney cell line transfected with eGFP was stained with either (A) Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42401) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in GFP-positive cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in GFP-positive cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />