EGFR Antibodies
EGFR (Epidermal growth factor receptor), also known as ErbB1 and HER1, is a type I glycoprotein that belongs the ErbB subfamily of receptor tyrosine kinases (RTKs). EGFR functions in epithelial cell development and homeostasis and as a driver of tumorigenesis in cancer. The human EGFR is protein 1210 amino acids (aa) in length with a theoretical molecular weight (MW) of 134 kDa. We offer EGFR antibodies featured in 50+ publications, including multiple 4- and 5-star customer reviewed antibodies. Browse our inventory of recombinant, monoclonal, polyclonal, and EGFR-conjugated antibodies. Validated research applications for these anti-EGFR antibodies include Blocking/Neutralizing, CyTOF-ready, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC, Immunoprecipitation, Simple Western, Western Blot. We also offer Knockout (KO) validated EGFR antibodies. Our EGFR antibodies are reactive in Human, Mouse, Rat, Porcine, and more. Each antibody is fully covered by Novus’ Guarantee+. Choose the anti-EGFR antibody best suited for your research needs by viewing the technical details, images, references, and related products.
Alternate Names for EGFR Antibodies
anti-avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, anti-cell growth inhibiting protein 40 antibody, anti-cell proliferation-inducing protein 61 antibody, anti-EC 2.7.10 antibody, anti-EC 2.7.10.1 antibody, anti-EGF R antibody, anti-EGFR antibody, anti-epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog) antibody, anti-epidermal growth factor receptor antibody, anti-ErbB antibody, anti-ErbB1 antibody, anti-ERBB1PIG61 antibody, anti-HER1 antibody, anti-HER-1 antibody, anti-mENA antibody, anti-Proto-oncogene c-ErbB-1 antibody, anti-Receptor tyrosine-protein kinase erbB-1 antibody
119 Results for "egfr" in Primary Antibodies
![IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123725..jpg "IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![IHC-P-EGFR Antibody [NBP3-03213] -Human placenta using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123838..jpg)
![IHC-P-EGFR Antibody [NBP3-03213] - Mouse heart using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123952..jpg)
![Western Blot: EGFR Antibody [NBP3-03213] - analysis of A431, using EGFR Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-western-blot-611202384024..jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP3-03213] - Analysis of A-431 cells using EGFR Rabbit pAb at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunocytochemistry-immunofluorescence-611202384153..jpg)
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0003.jpg "Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.")
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Staining of A431 cells with RAT ANTI HUMAN EGF RECEPTOR (NB 600-724) visualised with F(ab')2 RABBIT ANTI RAT IgG: FITC secondary.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0001.jpg)
![Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Western-Blot-NBP2-34243-img0006.jpg "Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).")
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0001.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0002.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Electropherogram image of the corresponding Simple Western lane. EGF R/ErbB1 antibody was used at 10 ug/ml dilution of A431 and HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0004.jpg)
![Protein Array: EGFR Antibody (H9B4) [NBP2-34243] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (H9B4) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Protein-Array-NBP2-34243-img0005.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining in human placenta and pancreas tissues . Corresponding EGFR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining in human placenta and pancreas tissues . Corresponding EGFR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human testis shows no in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0009.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0006.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0008.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29820241420932.jpg "Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: EGFR Antibody (PD00-75) [NBP3-32255] - Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32255) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-2920241192377.jpg)
![Flow Cytometry: EGFR Antibody (PD00-75) [NBP3-32255] - Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32255, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29202416372141.jpg)
![Western Blot: EGFR Antibody (PD00-75) [NBP3-32255] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32255) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MDA-MB-468 cell lysate Lane 4: MCF7 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-3920248485012.jpg)
