DPPIV/CD26 Antibodies: Novus Biologicals

AF1180

DPPIV/CD26 Antibody [Unconjugated]

(2 Reviews)

100 μg

AF1180

HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Expression of different markers in the eight human colon cancer cell lines analyzed. (A) Western blot analysis of EpCAM, LGR5, CD26, E-cadherin and vimentin expression in total cell extracts from the eight cell lines (20 μg of protein in each line). Data shown are representative of three experiments. (B) E-cadherin and EpCAM expression analysis by immunofluorescence in HT-29 and Caco-2 cells. (C) CD44 and CD26 expression analysis by immunofluorescence in HT-29 and Caco-2 cells. (D) LGR5 expression analysis by immunofluorescence in DLD-1 and Caco-2 cells. Nuclei were stained with DAPI. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31285270), licensed under a CC-BY license. Not internally tested by R&D Systems.

Comparison of histone modifier sets between two hepatic cell cultures.(A) Differentiation scheme of hESC towards Hepatocyte-like cells (Hep-like). (B) Hep-like cells were generated from hESC and stained with antibodies specific for DPP4 and albumin. Nuclei were stained with the DNA dye H-33342 (blue). Scale bars: 100 µm. (C) RT-qPCR data from three independent experiments for hepatic (ALB, CYP3A, CYP7A1, DPP4, HNF4, MET) and neuronal (TH, DCX, TUBB3) markers. Relative gene expression was calculated using hESC as a calibrator and a set of three reference genes (HPRT, RPL13A, GAPDH). (D) Transcript levels of epigenetic modifiers were measured by RT-qPCR in human liver (huHep), Hep-like islets (Hep-like) and embryonic stem cells (hESC). Data for huHep and Hep-like are indicated as relative change compared to hESC (as reference cell). For comparative display, a scatter plot was constructed so that differentially expressed genes that show pos. association (between Hep-like and huHep) are found in red fields, and those that differed in the sense of regulation fall into blue fields. Values of >10 were set to 10. For quadrant count ratio analysis (QCR) only expression values >2 or <−2 were included. (E) The data measured in D were plotted as heat map, sorted according to rel. huHep expression levels. Transcripts that were >2-fold higher expressed in tissue than in hESC are marked in red, >2-fold lower expression is marked in blue. The color scale ranges from a fold regulation of −20 (dark blue) to +20 (dark red). Measures of variance and p-values are indicated in the supplemental material, genes not regulated significantly (vs. hESC) are displayed as “n.s.”. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0102035), licensed under a CC-BY license. Not internally tested by R&D Systems.

Nuclear Translocation of Antitumor CD26 mAbs in Cancer Cells. (D) Jurkat/mock or Jurkat/CD26 cells incubated with biotin-labeled control IgG1 or 1F7 for 1 hour. Nuclear (Nuc)&membrane (Mem) extracts of these cells pulled-down with Neutravidin,&then subjected to immunoblot analysis using streptavidin or antibodies to CD26 or Na+/K+ ATPase (membrane marker). HC, heavy chain; LC, light chain. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23638030), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB1180) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Equine IL-4 was serially diluted and captured by Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # <a class=

MAB1180) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1180U-100UG
Alexa Fluor 405	AF1180V-100UG
Alexa Fluor 488	AF1180G-100UG
Alexa Fluor 532	AF1180X-100UG
Alexa Fluor 594	AF1180T-100UG
Alexa Fluor 647	AF1180R-100UG
Alexa Fluor 700	AF1180N-100UG
Alexa Fluor 750	AF1180S-100UG
Alexa Fluor Plus 405	AF1180AFP405-100UG
Alexa Fluor Plus 488	AF1180AFP488-100UG
Alexa Fluor Plus 555	AF1180AFP555-100UG
Alexa Fluor Plus 594	AF1180AFP594-100UG
Alexa Fluor Plus 647	AF1180AFP647-100UG
Alexa Fluor Plus 680	AF1180AFP680-100UG
Allophycocyanin	AF1180A
Biotin	BAF1180
CoraFluor 1	AF1180CL1
DyLight 350	AF1180D
DyLight 405	AF1180E
DyLight 488	AF1180K
DyLight 550	AF1180L
DyLight 594	AF1180M
DyLight 650	AF1180W
DyLight 680	AF1180Y
DyLight 755	AF1180Z
FITC	AF1180F
HRP	AF1180H
Janelia Fluor 525	AF1180JF525
Janelia Fluor 549	AF1180I
Janelia Fluor 646	AF1180J
Janelia Fluor 669	AF1180JF669
PE	AF1180P
PerCP	AF1180C
mFluor Violet 450 SE	AF1180MFV450
mFluor Violet 500 SE	AF1180MFV500
mFluor Violet 610 SE	AF1180MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1180-SP

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AF954

DPPIV/CD26 Antibody [Unconjugated]

(44 Publications)

100 μg

AF954

HAF019). A specific band was detected for DPPIV/CD26 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse thymus tissue and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for DPPIV/CD26 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse thymus tissue and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DPPIV/CD26 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

DPPIV/CD26 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of rat lung tissue and mouse thymus tissue, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 144-169 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

siRNA-mediated knock-down of hepatic dpp4 in db/db mice.(A) Schematic illustration of the study design. (B, C and D) Liver samples were taken after study termination at day 30. (B) dpp4 mRNA expression was analyzed by real-time PCR after indicated treatment. (C) Quantification of dpp4 protein abundance. (D) Representative Western Blot images show dpp4 protein levels after indicated treatment. (E) Circulating dpp4 protein levels as well as (F) circulating dpp4 activity was measured three days after last dosing at day 30. (G) Quantification of active GLP-1 and (H) GIP at day 30. Data are mean values ± SEM, n = 6–8, *p<0.05, **p<0.005 and ***p<0.0001 as indicated (ANOVA, Tukey's multiple comparisons test). FC = fold change. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31794591), licensed under a CC-BY license. Not internally tested by R&D Systems.

Linagliptin promotes NO-mediated endothelial-cardiomyocyte communication. (A) Immunoblotting showing absence of DPP-4 in primary cultured mouse cardiomyocytes (mouse duodenum = positive control). (B) Immunoblotting C166 endothelial cell lysates for DPP-4 (1 and 2 are replicates). (C and D) Immunoblotting C166 mouse endothelial cells for phospho-Akt (Ser473) (C) or phospho-eNOS (Ser1177) (D) after incubation with 9 nmol/L linagliptin for 5 min (control, n = 8, linagliptin, n = 6). (E) Immunoblotting adult mouse cardiomyocytes for PLN(Ser16) after exposure to 100 µmol/L S-nitroso-N-acetylpenicillamine (SNAP) for 5 min (vehicle, n = 6; SNAP, n = 7). (F) cGMP concentration in adult mouse cardiomyocytes under control conditions or after incubation with 9 nmol/L linagliptin for 5 min (n = 4/condition). (G) (Left) Co-culture experiment in which adult mouse cardiomyocytes were cultured in the presence of C166 endothelial cells in cell culture inserts before the addition of linagliptin. (Right) cGMP concentration in adult mouse cardiomyocytes co-cultured with C166 endothelial cells under control conditions or after incubation with 9 nmol/L linagliptin for 5 min. Values are mean ± S.D.. * p < 0.05, ** p < 0.01 by the two-tailed Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32796688), licensed under a CC-BY license. Not internally tested by R&D Systems.

At human Cmax equivalent, linagliptin indirectly increases Ser16 phosphorylation of the Ca2+ handling protein phospholamban in cardiomyocytes. (A) Immunoblotting for serine 16 phosphorylated phospholamban (PLN(Ser16)) in heart homogenates of young, non-diabetic adult mice perfused with Krebs Henseleit buffer (KHB) or KHB supplemented with 9 nmol/L linagliptin and subjected to 20 min no-flow ischemia followed by 40 min reperfusion (n = 6/group). (B) Immunoblotting primary cultured mouse cardiomyocytes for PLN(Ser16) under control conditions or after incubation with 9 nmol/L linagliptin for 5 min (n = 13/condition). (C) Serial cut sections from a C57BL/6 mouse heart immunostained for DPP-4 or CD31 showing predominant DPP-4 expression in CD31+ cells indicative of endothelial expression (n = 4). Scale bar = 50 µm. (D–F) Volcano plots of differentially expressed genes determined by RNA sequencing of adult mouse cardiomyocytes derived from young, non-diabetic, non-high fat diet-fed mice (D; n = 5/condition) or aged, diabetic, high fat diet-fed (DM-HFD) mice (E; n = 3/condition) exposed to linagliptin (9 nmol/L) or vehicle for 24 h. (F) Volcano plot of differentially expressed genes in vehicle-treated cardiomyocytes derived from DM-HFD mice (n = 3) and control mice (n = 5). Values are mean ± S.D.. *p < 0.05 by two-tailed Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32796688), licensed under a CC-BY license. Not internally tested by R&D Systems.

954-SE) was serially diluted and captured by Rat Anti-Mouse DPPIV/CD26 Monoclonal Antibody (Catalog # MAB954) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse DPPIV/CD26 (Catalog # <a class=

954-SE) was serially diluted and captured by Rat Anti-Mouse DPPIV/CD26 Monoclonal Antibody (Catalog # MAB954) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF954U-100UG
Alexa Fluor 405	AF954V-100UG
Alexa Fluor 488	AF954G-100UG
Alexa Fluor 532	AF954X-100UG
Alexa Fluor 594	AF954T-100UG
Alexa Fluor 647	AF954R-100UG
Alexa Fluor 700	AF954N-100UG
Alexa Fluor 750	AF954S-100UG
Alexa Fluor Plus 405	AF954AFP405-100UG
Alexa Fluor Plus 488	AF954AFP488-100UG
Alexa Fluor Plus 555	AF954AFP555-100UG
Alexa Fluor Plus 594	AF954AFP594-100UG
Alexa Fluor Plus 647	AF954AFP647-100UG
Alexa Fluor Plus 680	AF954AFP680-100UG
Allophycocyanin	AF954A
Biotin	BAF954
CoraFluor 1	AF954CL1
DyLight 350	AF954D
DyLight 405	AF954E
DyLight 488	AF954K
DyLight 550	AF954L
DyLight 594	AF954M
DyLight 650	AF954W
DyLight 680	AF954Y
DyLight 755	AF954Z
FITC	AF954F
HRP	AF954H
Janelia Fluor 525	AF954JF525
Janelia Fluor 549	AF954I
Janelia Fluor 646	AF954J
Janelia Fluor 669	AF954JF669
PE	AF954P
PerCP	AF954C
mFluor Violet 450 SE	AF954MFV450
mFluor Violet 500 SE	AF954MFV500
mFluor Violet 610 SE	AF954MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF954-SP

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MAB1180

DPPIV/CD26 Antibody (222113) [Unconjugated]

(8 Publications)

100 μg

MAB1180

HAF005). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB006, open histogram) followed by Anti-mouse PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human peripheral blood lymphocytes were stained with Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180, filled histogram) or isotype control antibody (Catalog # <a class=

MAB006, open histogram) followed by Anti-mouse PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

DY990). Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Human DPPIV/CD26 was serially diluted and captured by Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180) coated on a Clear Polystyrene Microplate (Catalog # <a class=

DY990). Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1180U-100UG
Alexa Fluor 405	FAB1180V-100UG
Alexa Fluor 488	FAB1180G-100UG
Alexa Fluor 532	FAB1180X
Alexa Fluor 594	FAB1180T-100UG
Alexa Fluor 647	FAB1180R-100UG
Alexa Fluor 700	FAB1180N-100UG
Alexa Fluor 750	FAB1180S-100UG
Alexa Fluor Plus 405	FAB1180AFP405-100UG
Alexa Fluor Plus 488	FAB1180AFP488-100UG
Alexa Fluor Plus 555	FAB1180AFP555-100UG
Alexa Fluor Plus 594	FAB1180AFP594-100UG
Alexa Fluor Plus 647	FAB1180AFP647-100UG
Alexa Fluor Plus 680	FAB1180AFP680-100UG
Allophycocyanin	FAB1180A
Allophycocyanin/Cy7	FAB1180APCCY7
Biotin	FAB1180B
CoraFluor 1	FAB1180CL1
DyLight 350	FAB1180D
DyLight 405	FAB1180E
DyLight 488	FAB1180K
DyLight 550	FAB1180L
DyLight 594	FAB1180M
DyLight 650	FAB1180W
DyLight 680	FAB1180Y
DyLight 755	FAB1180Z
HRP	FAB1180H
Janelia Fluor 525	FAB1180JF525
Janelia Fluor 549	FAB1180I
Janelia Fluor 646	FAB1180J
Janelia Fluor 669	FAB1180JF669
PE/Atto594	FAB1180PEATT594
PE/Cy5.5	FAB1180PECY55
PE/Cy7	FAB1180PECY7
PerCP	FAB1180C
mFluor Violet 450 SE	FAB1180MFV450
mFluor Violet 500 SE	FAB1180MFV500
mFluor Violet 610 SE	FAB1180MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1180-SP
Carrier Free	MAB1180-500
Carrier Free	FAB1180V-100UG
Carrier Free	FAB1180U-100UG
Carrier Free	FAB1180T-100UG
Carrier Free	FAB1180S-100UG
Carrier Free	FAB1180R-100UG
Carrier Free	FAB1180N-100UG
Carrier Free	FAB1180G-100UG

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NB100-59021

DPPIV/CD26 Antibody - BSA Free

(9 Publications)

0.05 mg

NB100-59021

DPPIV-CD26-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59021-img0003.jpg

DPPIV-CD26-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59021-img0004.jpg

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody - BSA Free [NB100-59021] - Immunohistochemical analysis of human prostate.

Reactivity:

Hu, Pm, Pm, Pm-Or

Species Glossary

Reactivity: Hu, Pm, Pm, Pm-Or

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-78791

DPPIV/CD26 Antibody (JM11-42)

100 ul

NBP2-78791

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. Counter stained with hematoxylin.

Flow Cytometry: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of Hela cells with CD26 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. Counter stained with hematoxylin.

<b>Genetic Strategies Validation. </b>Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - All lanes: Western blot analysis of CD26 with anti-CD26 antibody at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2: CD26 knockout Hela whole cell lysate (10 ug).NBP2-78791 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-02154

DPPIV/CD26 Antibody (OTI11D7)

(1 Publication)

0.1 ml

NBP2-02154

Immunohistochemistry: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Lungs harvested from BALB/c mice at day 3. Dilution: 1:800

Flow Cytometry: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Analysis of Hela cells, using anti-CD26 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western Blot: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD26 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD26.

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Staining of paraffin-embedded liver tissue using anti-CD26 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70588AF350
Alexa Fluor 405	NBP2-70588AF405
Alexa Fluor 488	NBP2-70588AF488
Alexa Fluor 532	NBP2-70588AF532
Alexa Fluor 594	NBP2-70588AF594
Alexa Fluor 647	NBP2-70588AF647
Alexa Fluor 700	NBP2-70588AF700
Alexa Fluor 750	NBP2-70588AF750
Alexa Fluor Plus 405	NBP2-70588AFP405
Alexa Fluor Plus 488	NBP2-70588AFP488
Alexa Fluor Plus 555	NBP2-70588AFP555
Alexa Fluor Plus 594	NBP2-70588AFP594
Alexa Fluor Plus 647	NBP2-70588AFP647
Alexa Fluor Plus 680	NBP2-70588AFP680
Allophycocyanin	NBP2-70588APC
Biotin	NBP2-70588B
CoraFluor 1	NBP2-70588CL1
DyLight 350	NBP2-70588UV
DyLight 405	NBP2-70588V
DyLight 488	NBP2-70588G
DyLight 550	NBP2-70588R
DyLight 594	NBP2-70588DL594
DyLight 650	NBP2-70588C
DyLight 680	NBP2-70588FR
DyLight 755	NBP2-70588IR
FITC	NBP2-70588F
HRP	NBP2-70588H
Janelia Fluor 525	NBP2-70588JF525
Janelia Fluor 549	NBP2-70588JF549
Janelia Fluor 646	NBP2-70588JF646
Janelia Fluor 669	NBP2-70588JF669
PE	NBP2-70588PE
PerCP	NBP2-70588PCP
mFluor Violet 450 SE	NBP2-70588MFV450
mFluor Violet 500 SE	NBP2-70588MFV500
mFluor Violet 610 SE	NBP2-70588MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70588

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NBP2-22434

DPPIV/CD26 Antibody (236.3) - Azide and BSA Free

500 μg

NBP2-22434

Flow Cytometry: DPPIV/CD26 Antibody (236.3) [NBP2-22434] - Analysis of CD26 in PC12 cells (green) compared to an isotype control (blue).

Flow Cytometry: DPPIV/CD26 Antibody (236.3) [NBP2-22434] - Analysis of CD26 in Hela cells (green) compared to an isotype control (blue).

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22434AF350
Alexa Fluor 532	NBP2-22434AF532
Alexa Fluor 594	NBP2-22434AF594
Alexa Fluor 750	NBP2-22434AF750
Alexa Fluor Plus 405	NBP2-22434AFP405
Alexa Fluor Plus 488	NBP2-22434AFP488
Alexa Fluor Plus 555	NBP2-22434AFP555
Alexa Fluor Plus 594	NBP2-22434AFP594
Alexa Fluor Plus 647	NBP2-22434AFP647
Alexa Fluor Plus 680	NBP2-22434AFP680
CoraFluor 1	NBP2-22434CL1
DyLight 594	NBP2-22434DL594
Janelia Fluor 525	NBP2-22434JF525
Janelia Fluor 549	NBP2-22434JF549
Janelia Fluor 646	NBP2-22434JF646
Janelia Fluor 669	NBP2-22434JF669
mFluor Violet 450 SE	NBP2-22434MFV450
mFluor Violet 500 SE	NBP2-22434MFV500
mFluor Violet 610 SE	NBP2-22434MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-22434-100ug

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MAB954

DPPIV/CD26 Antibody (155211) [Unconjugated]

500 μg

MAB954

954-SE) was serially diluted and captured by Rat Anti-Mouse DPPIV/CD26 Monoclonal Antibody (Catalog # MAB954) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

954-SE) was serially diluted and captured by Rat Anti-Mouse DPPIV/CD26 Monoclonal Antibody (Catalog # MAB954) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse DPPIV/CD26 (Catalog # 954-SE) was serially diluted and captured by Rat Anti-Mouse DPPIV/CD26 Monoclonal Antibody (Catalog # MAB954) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB954U-100UG
Alexa Fluor 405	FAB954V-100UG
Alexa Fluor 488	FAB954G-100UG
Alexa Fluor 532	FAB954X-100UG
Alexa Fluor 594	FAB954T-100UG
Alexa Fluor 647	FAB954R-100UG
Alexa Fluor 700	FAB954N-100UG
Alexa Fluor 750	FAB954S-100UG
Alexa Fluor Plus 405	FAB954AFP405-100UG
Alexa Fluor Plus 488	FAB954AFP488-100UG
Alexa Fluor Plus 555	FAB954AFP555-100UG
Alexa Fluor Plus 594	FAB954AFP594-100UG
Alexa Fluor Plus 647	FAB954AFP647-100UG
Alexa Fluor Plus 680	FAB954AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB954-SP

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NB100-61658

DPPIV/CD26 Antibody

(3 Publications)

0.1 mg

NB100-61658

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody [NB100-61658] - Staining of paraffin embedded Human Prostate with antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Reactivity:

Hu, Rt, Mu

Species Glossary

Reactivity: Hu, Rt, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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MAB9541

DPPIV/CD26 Antibody (155202) [Unconjugated]

(1 Publication)

500 μg

MAB9541

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_lightbox">

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="Mouse splenocytes were stained with Rat Anti-Mouse DPPIV/CD26 Monoclonal Antibody (Catalog # MAB9541, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9541U-100UG
Alexa Fluor 405	FAB9541V-100UG
Alexa Fluor 488	FAB9541G-100UG
Alexa Fluor 532	FAB9541X
Alexa Fluor 594	FAB9541T-100UG
Alexa Fluor 647	FAB9541R-100UG
Alexa Fluor 700	FAB9541N-100UG
Alexa Fluor 750	FAB9541S-100UG
Alexa Fluor Plus 405	FAB9541AFP405-100UG
Alexa Fluor Plus 488	FAB9541AFP488-100UG
Alexa Fluor Plus 555	FAB9541AFP555-100UG
Alexa Fluor Plus 594	FAB9541AFP594-100UG
Alexa Fluor Plus 647	FAB9541AFP647-100UG
Alexa Fluor Plus 680	FAB9541AFP680-100UG
Allophycocyanin/Cy7	FAB9541APCCY7
Biotin	FAB9541B
CoraFluor 1	FAB9541CL1
DyLight 350	FAB9541D
DyLight 405	FAB9541E
DyLight 488	FAB9541K
DyLight 550	FAB9541L
DyLight 594	FAB9541M
DyLight 650	FAB9541W
DyLight 680	FAB9541Y
DyLight 755	FAB9541Z
HRP	FAB9541H
Janelia Fluor 525	FAB9541JF525
Janelia Fluor 549	FAB9541I
Janelia Fluor 646	FAB9541J
Janelia Fluor 669	FAB9541JF669
PE/Atto594	FAB9541PEATT594
PE/Cy5.5	FAB9541PECY55
PE/Cy7	FAB9541PECY7
PerCP	FAB9541C
mFluor Violet 450 SE	FAB9541MFV450
mFluor Violet 500 SE	FAB9541MFV500
mFluor Violet 610 SE	FAB9541MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9541-SP
Carrier Free	FAB9541V-100UG
Carrier Free	FAB9541U-100UG
Carrier Free	FAB9541T-100UG
Carrier Free	FAB9541S-100UG
Carrier Free	FAB9541R-100UG
Carrier Free	FAB9541N-100UG
Carrier Free	FAB9541G-100UG

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MAB11664

DPPIV/CD26 Antibody (1095112) [Unconjugated]

100 μg

MAB11664

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of lymphatic endothelial cells. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD26/DPP4 Antibody was detected in immersion fixed paraffin-embedded sections of human Prostate Cancer using Mouse Anti-Human CD26/DPP4, Monoclonal Antibody (Catalog #MAB11678) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of lymphatic endothelial cells. Protocol available in COMET™ Panel Builder." alt="CD26/DPP4 Antibody was detected in immersion fixed paraffin-embedded sections of human Prostate Cancer using Mouse Anti-Human CD26/DPP4, Monoclonal Antibody (Catalog #MAB11678) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of lymphatic endothelial cells. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of lymphatic vessels. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Mouse Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB11664) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of lymphatic vessels. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11664U-100UG
Alexa Fluor 405	FAB11664V-100UG
Alexa Fluor 488	FAB11664G-100UG
Alexa Fluor 532	FAB11664X-100UG
Alexa Fluor 594	FAB11664T-100UG
Alexa Fluor 647	FAB11664R-100UG
Alexa Fluor 700	FAB11664N-100UG
Alexa Fluor 750	FAB11664S-100UG
Alexa Fluor Plus 405	FAB11664AFP405-100UG
Alexa Fluor Plus 488	FAB11664AFP488-100UG
Alexa Fluor Plus 555	FAB11664AFP555-100UG
Alexa Fluor Plus 594	FAB11664AFP594-100UG
Alexa Fluor Plus 647	FAB11664AFP647-100UG
Alexa Fluor Plus 680	FAB11664AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11664-SP

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NBP3-16980

DPPIV/CD26 Antibody - BSA Free

100 ul

NBP3-16980

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody [NBP3-16980] - Analysis in human parathyroid gland and testis tissues using Anti-DPP4 antibody. Corresponding DPP4 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody [NBP3-16980] - Staining of human parathyroid gland shows high expression.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

DPPIV/CD26 Antibodies

DPPIV/CD26 Antibody [Unconjugated]

DPPIV/CD26 Antibody [Unconjugated]

DPPIV/CD26 Antibody (222113) [Unconjugated]

DPPIV/CD26 Antibody - BSA Free

DPPIV/CD26 Antibody (JM11-42)

DPPIV/CD26 Antibody (OTI11D7)

DPPIV/CD26 Antibody (236.3) - Azide and BSA Free

DPPIV/CD26 Antibody (155211) [Unconjugated]

DPPIV/CD26 Antibody

DPPIV/CD26 Antibody (155202) [Unconjugated]

DPPIV/CD26 Antibody (1095112) [Unconjugated]

DPPIV/CD26 Antibody - BSA Free