MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="Human DC-SIGN and DC-SIGNR transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN+ DC-SIGNR Monoclonal Antibody (Catalog # MAB1621, filled histo­grams) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

FAB141F).Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003)." class="big_lightbox"> FAB141F).Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003)." class="big_thumb" />

CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF016). A specific band was detected for SIGNR3/CD209d at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for SIGNR3/CD209d at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse SIGNR3/CD209d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SIGNR3/CD209d at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Immunoblot Buffer Group 1." class="big_lightbox"> Immunoblot Buffer Group 1." alt="Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat SIGNR3/CD209d Monoclonal Antibody (Catalog # MAB7220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for SIGNR3/CD209d at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />