| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37975-25ul | |||
HAF016). A specific band was detected for CARD9 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CARD9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5248) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CARD9 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5248-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76679-0.025mg | |||
![Western Blot: CARD9 Antibody [NBP1-32982] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-NBP1-32982-img0004.jpg "Western Blot: CARD9 Antibody [NBP1-32982] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP1-32982] - H1299 xenograft, using CARD9 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP1-32982-img0002.jpg)
![Western Blot: CARD9 Antibody [NBP1-32982] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-NBP1-32982-img0003.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Analysis in human spleen and cerebral cortex tissues. Corresponding CARD9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Analysis in human spleen and cerebral cortex tissues. Corresponding CARD9 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human spleen shows weak to moderate cytoplasmic positivity in a subset of leukocytes.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0009.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0006.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human tonsil shows moderate cytoplasmic positivity in a subset of leukocytes.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0007.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0008.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: CARD9 Antibody (PSH0-41) [NBP3-32106] - Immunocytochemistry analysis of THP-1 cells labeling CARD9 with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32106_rabbit-card9-mab-psh0-41-29202410334029.jpg "Immunocytochemistry/ Immunofluorescence: CARD9 Antibody (PSH0-41) [NBP3-32106] - Immunocytochemistry analysis of THP-1 cells labeling CARD9 with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CARD9 Antibody (PSH0-41) [NBP3-32106] - Flow cytometric analysis of THP-1 cells labeling CARD9.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32106, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32106_rabbit-card9-mab-psh0-41-29202416372116.jpg)
![Western Blot: CARD9 Antibody [NBP1-76679] - Analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 ug /ml.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-NBP1-76679-img0004.jpg "Western Blot: CARD9 Antibody [NBP1-76679] - Analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 ug /ml.")
![Immunocytochemistry/ Immunofluorescence: CARD9 Antibody - BSA Free [NBP1-76679] - Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76679_rabbit-polyclonal-card9-antibody-271120242042575.jpg)
![Western Blot: CARD9 Antibody [H00064170-B01P] - Analysis of CARD9 expression in transfected 293T cell line by CARD9 polyclonal antibody. Lane 1: CARD9 transfected lysate(54.12 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-H00064170-B01P-img0002.jpg "Western Blot: CARD9 Antibody [H00064170-B01P] - Analysis of CARD9 expression in transfected 293T cell line by CARD9 polyclonal antibody. Lane 1: CARD9 transfected lysate(54.12 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CARD9 Antibody [H00064170-B01P] - Analysis of purified antibody to CARD9 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunocytochemistry-Immunofluorescence-H00064170-B01P-img0001.jpg)
![Immunoprecipitation:CARD9 AntibodyNBP3-30275] -Detection of human CARD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARD9 antibody (NBP3-30275 lot 2) used for IP at 1 µg per reaction. CARD9 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30275-1). For blotting immunoprecipitated CARD9, NBP3-30275 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30275_rabbit-card9-pab-301220241556511.jpg "Immunoprecipitation:CARD9 AntibodyNBP3-30275] -Detection of human CARD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARD9 antibody (NBP3-30275 lot 2) used for IP at 1 µg per reaction. CARD9 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30275-1). For blotting immunoprecipitated CARD9, NBP3-30275 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: CARD9 Antibody (S02-7H5) [NBP3-19592] - Western blot detection of CARD9 in K562, THP-1 cell lysates using NBP3-19592 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 62kDa.](http://images.novusbio.com/fullsize/CARD9-Antibody-S02-7H5-Western-Blot-NBP3-19592-img0001.jpg "Western Blot: CARD9 Antibody (S02-7H5) [NBP3-19592] - Western blot detection of CARD9 in K562, THP-1 cell lysates using NBP3-19592 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 62kDa.")
