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![Western Blot: IL-12 p70/IL-12A Antibody [NBP1-78112] - OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (diluted to 1X) overnight at 4 degrees C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the imaging system](http://images.novusbio.com/fullsize/IL-12-p70-IL-12A-Antibody-Western-Blot-NBP1-78112-img0003.jpg "Western Blot: IL-12 p70/IL-12A Antibody [NBP1-78112] - OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (diluted to 1X) overnight at 4 degrees C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the imaging system")
![Western Blot: p35 Antibody [NBP2-21650] - Detection of 0.1 ug of recombinant p35 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.](http://images.novusbio.com/fullsize/p35-Antibody-Western-Blot-NBP2-21650-img0001.jpg "Western Blot: p35 Antibody [NBP2-21650] - Detection of 0.1 ug of recombinant p35 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.")
![Western Blot: OspC Antibody [NBP2-21683] - Detection of 0.1 ug of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.](http://images.novusbio.com/fullsize/OspC-Antibody-Western-Blot-NBP2-21683-img0002.jpg "Western Blot: OspC Antibody [NBP2-21683] - Detection of 0.1 ug of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.")
![Western Blot: CRASP-2 Antibody [NBP2-21651] - Detection of 0.1 ug of recombinant CRASP-2 protein. Lane 1: Molecular weight markers. Lane 2: MBP-CRASP-2 fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.](http://images.novusbio.com/fullsize/CRASP-2-Antibody-Western-Blot-NBP2-21651-img0001.jpg "Western Blot: CRASP-2 Antibody [NBP2-21651] - Detection of 0.1 ug of recombinant CRASP-2 protein. Lane 1: Molecular weight markers. Lane 2: MBP-CRASP-2 fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.")
![Western Blot: ErpN/OspE Antibody [NBP2-21682] - Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein. Lane 3: fusion protein plus cleaved fusion proteins. Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in Lane 3 are fusion protein, or breakdown products of the fusion protein. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.](http://images.novusbio.com/fullsize/ErpN-OspE-Antibody-Western-Blot-NBP2-21682-img0001.jpg "Western Blot: ErpN/OspE Antibody [NBP2-21682] - Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein. Lane 3: fusion protein plus cleaved fusion proteins. Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in Lane 3 are fusion protein, or breakdown products of the fusion protein. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.")
![Western Blot: VlsE Antibody [NBP2-21653] - Detection of 0.1 ug of recombinant VlsE protein. Lane 1: Molecular weight markers. Lane 2: MBP-VlsE fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.](http://images.novusbio.com/fullsize/VlsE-Antibody-Western-Blot-NBP2-21653-img0001.jpg "Western Blot: VlsE Antibody [NBP2-21653] - Detection of 0.1 ug of recombinant VlsE protein. Lane 1: Molecular weight markers. Lane 2: MBP-VlsE fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.")
![Western Blot: ErpD/Arp37 Antibody [NBP2-21681] - Lane 1: Molecular weight markers. Lane 2: MBP-ErpD/Arp37 fusion proteins. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.](http://images.novusbio.com/fullsize/ErpD-Arp37-Antibody-Western-Blot-NBP2-21681-img0001.jpg "Western Blot: ErpD/Arp37 Antibody [NBP2-21681] - Lane 1: Molecular weight markers. Lane 2: MBP-ErpD/Arp37 fusion proteins. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.")
![Western Blot: Surface Lipoprotein p27 Antibody [NBP2-21652] - Detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.](http://images.novusbio.com/fullsize/Surface-Lipoprotein-p27-Antibody-Western-Blot-NBP2-21652-img0001.jpg "Western Blot: Surface Lipoprotein p27 Antibody [NBP2-21652] - Detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in Blocking Buffer and imaged on the 4000 imaging system.")