ASXL1 Antibodies
We offer ASXL1 Antibodies for use in common research applications: ELISA, Sandwich ELISA, Western Blot. Each ASXL1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ASXL1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the ASXL1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ASXL1 polyclonal antibodies and browse our ASXL1 monoclonal antibody catalog.
Alternate Names for ASXL1 Antibodies
anti-ASXL1 antibody, anti-additional sex combs like 1 (Drosophila) antibody
2 Results for "asxl1" in Primary Antibodies
![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction. Evaluation of ASXL1 protein expression by Western blotting. Left-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), and KBM5 clones (labeled 1-5) with heterozygous precise correction of the ASXL1 mutation. Right-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, and KBM5 clones (labeled 1-5) with homozygous precise correction of the ASXL1 mutation. Beta-actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6392) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ASXL1-Antibody-6E2-Western-Blot-H00171023-M05-img0006.jpg "Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction. Evaluation of ASXL1 protein expression by Western blotting. Left-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), and KBM5 clones (labeled 1-5) with heterozygous precise correction of the ASXL1 mutation. Right-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, and KBM5 clones (labeled 1-5) with homozygous precise correction of the ASXL1 mutation. Beta-actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6392) licensed under a CC-BY license.")
![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Analysis of ASXL1 expression in MCF-7 (Cat # L046V1).](http://images.novusbio.com/fullsize/ASXL1-Antibody-6E2-Western-Blot-H00171023-M05-img0004.jpg)
![ELISA: ASXL1 Antibody (6E2) [H00171023-M05] - Detection limit for recombinant GST tagged ASXL1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ASXL1-Antibody-6E2-ELISA-H00171023-M05-img0005.jpg)
![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction(A) Evaluation of ASXL1 protein expression by WB. Left: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), & KBM5 clones (labeled 1–5) w/ heterozygous precise correction of ASXL1 mutation. Right: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & KBM5 clones (labeled 1–5) w/ homozygous precise correction of ASXL1 mutation. beta -actin used as loading control. (B) Evaluation of expression levels of HOXA genes using quantitative real-time PCR (q-RT-PCR). Expression levels of HOXA5, 6, 7, 9, 10 & 13 measured in three ASXL1 homozygous corrected KBM5 clones compared w/ uncorrected cells. Results shown obtained from six independent experiments for each clone. Values in ASXL1 homozygous corrected cells are relative to uncorrected cells. Bar graphs show mean + standard error of mean (s.e.m.) (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, paired t-test). (C) Evaluation of H3K27me3 levels & expression of PRC2 components by WB in uncorrected KBM5 cells & three KBM5 clones w/ homozygous correction of ASXL1 mutation. H3K27me3 levels & total H3 levels evaluated using purified histone fractions. Expression levels of two PRC2 components (EZH2, SUZ12) determined using whole cell lysates. beta -actin used as loading control. (D) Immunoprecipitation of BAP1 in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & three KBM5 clones w/ homozygous correction of ASXL1 mutation. The BAP1 protein fraction immunoprecipitated using a BAP1 antibody & stained for ASXL1 & BAP1. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.6392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00171023-m05_mouse-monoclonal-asxl1-antibody-6e2-310202415485124.jpg)
![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction(A) Evaluation of ASXL1 protein expression by WB. Left: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), & KBM5 clones (labeled 1–5) w/ heterozygous precise correction of ASXL1 mutation. Right: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & KBM5 clones (labeled 1–5) w/ homozygous precise correction of ASXL1 mutation. beta -actin used as loading control. (B) Evaluation of expression levels of HOXA genes using quantitative real-time PCR (q-RT-PCR). Expression levels of HOXA5, 6, 7, 9, 10 & 13 measured in three ASXL1 homozygous corrected KBM5 clones compared w/ uncorrected cells. Results shown obtained from six independent experiments for each clone. Values in ASXL1 homozygous corrected cells are relative to uncorrected cells. Bar graphs show mean + standard error of mean (s.e.m.) (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, paired t-test). (C) Evaluation of H3K27me3 levels & expression of PRC2 components by WB in uncorrected KBM5 cells & three KBM5 clones w/ homozygous correction of ASXL1 mutation. H3K27me3 levels & total H3 levels evaluated using purified histone fractions. Expression levels of two PRC2 components (EZH2, SUZ12) determined using whole cell lysates. beta -actin used as loading control. (D) Immunoprecipitation of BAP1 in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & three KBM5 clones w/ homozygous correction of ASXL1 mutation. The BAP1 protein fraction immunoprecipitated using a BAP1 antibody & stained for ASXL1 & BAP1. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.6392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00171023-m05_mouse-monoclonal-asxl1-antibody-6e2-310202415482292.jpg)
![Western Blot: ASXL1 Antibody [NBP2-43807] - Analysis of A. 50 ug 293T whole cell lysate/extract B. 50 ug whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells ASXL1 antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/ASXL1-Antibody-Western-Blot-NBP2-43807-img0001.jpg "Western Blot: ASXL1 Antibody [NBP2-43807] - Analysis of A. 50 ug 293T whole cell lysate/extract B. 50 ug whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells ASXL1 antibody dilution: 1:5000.")
![Immunohistochemistry-Paraffin: ASXL1 Antibody [NBP2-43807] - Human esophagus carcinoma. ASXL1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ASXL1-Antibody-Immunohistochemistry-Paraffin-NBP2-43807-img0002.jpg)
![Western Blot: ASXL1 Antibody [NBP2-43807] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ASXL1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-43807_rabbit-polyclonal-asxl1-antibody-western-blot-2820237521..jpg)