ASC1 Antibodies
We offer ASC1 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each ASC1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ASC1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the ASC1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ASC1 polyclonal antibodies.
Alternate Names for ASC1 Antibodies
anti-ASC1 antibody, anti-TRIP4 antibody, anti-activating signal cointegrator 1 antibody, anti-ASC-1 antibody, anti-HsT17391 antibody, anti-thyroid hormone receptor interactor 4 antibody, anti-thyroid receptor interacting protein 4 antibody, anti-Thyroid receptor-interacting protein 4 antibody, anti-TR-interacting protein 4 antibody, anti-TRIP-4 antibody
7 Results for "asc1" in Primary Antibodies
![Western Blot: ASC1 Antibody [NBP1-89656] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ASC1-Antibody-Western-Blot-NBP1-89656-img0004.jpg "Western Blot: ASC1 Antibody [NBP1-89656] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: ASC1 Antibody [NBP1-89656] - Staining of human colon shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ASC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89656-img0005.jpg)
![Western Blot: ASC1 Antibody [NBP3-35533] - Western blot analysis of various lysates using ASC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-101220241139294.jpg "Western Blot: ASC1 Antibody [NBP3-35533] - Western blot analysis of various lysates using ASC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunoprecipitation: ASC1 Antibody [NBP3-35533] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells using 3 ug ASC1 antibody. Western blot was performed from the immunoprecipitate using ASC1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-1012202411392973.jpg)
![Immunohistochemistry: ASC1 Antibody [NBP3-35533] - Immunohistochemistry analysis of paraffin-embedded Rat heart using ASC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-1012202411343334.jpg)
![Immunohistochemistry: ASC1 Antibody [NBP3-35533] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ASC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-101220241143340.jpg)
![Immunohistochemistry: ASC1 Antibody [NBP3-35533] - Immunohistochemistry analysis of paraffin-embedded Human esophageal using ASC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-1012202411421060.jpg)
![Western Blot: ASC1 Antibody [NB100-419] - Detection of Human TRIP4/ASC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC1 antibody NB100-419 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASC1-Antibody-Western-Blot-NB100-419-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ASC1 Antibody [NB100-419] - Detection of Human TRIP4/ASC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC1 antibody NB100-419 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: ASC1 Antibody [NB100-419] - Detection of human ASC1 in Western Blot and immunoprecipitation, using NB100-419. Immunoprecipitated ASC1 was detected using an antibody to a distinctly different epitope on ASC1.](http://images.novusbio.com/fullsize/ASC1-Antibody-Western-Blot-NB100-419-img0001.jpg)
![Western Blot: ASC1 Antibody (PSH0-26) [NBP3-32049] - Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: U87-MG cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: HepG2 cell lysate Lane 7: RH-35 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32049) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32049_rabbit-asc1-mab-psh0-26-278202415212424.jpg "Western Blot: ASC1 Antibody (PSH0-26) [NBP3-32049] - Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: U87-MG cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: HepG2 cell lysate Lane 7: RH-35 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32049) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ASC1 Antibody (PSH0-26) [NBP3-32049] - Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32049) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32049_rabbit-asc1-mab-psh0-26-298202413585548.jpg)
![Immunoprecipitation:ASC1 AntibodyNBP3-30189] -Detection of human TRIP4/ASC-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (NBP3-30189 lot 4) used for IP at 6 µg per reaction. TRIP4/ASC-1 was also immunoprecipitated by a previous lot of this antibody (NBP3-30189 lot 3) and a second.inst a different epitope of TRIP4/ASC-1 (A300-203A). For blotting immunoprecipitated TRIP4/ASC-1, NBP3-30189 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30189_rabbit-asc1-pab-301220241226253.jpg "Immunoprecipitation:ASC1 AntibodyNBP3-30189] -Detection of human TRIP4/ASC-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (NBP3-30189 lot 4) used for IP at 6 µg per reaction. TRIP4/ASC-1 was also immunoprecipitated by a previous lot of this antibody (NBP3-30189 lot 3) and a second.inst a different epitope of TRIP4/ASC-1 (A300-203A). For blotting immunoprecipitated TRIP4/ASC-1, NBP3-30189 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: ASC1 Antibody [NBP2-58194] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize/ASC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58194-img0001.jpg "Immunocytochemistry/Immunofluorescence: ASC1 Antibody [NBP2-58194] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.")