Activin RIB/ALK-4 Antibodies

HAF017). A specific band was detected for Activin RIB/ALK-4 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Activin RIB/ALK-4 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Activin RIB/ALK-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF222) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Activin RIB/ALK-4 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for Activin RIB/ALK-4 at approximately 70 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Activin RIB/ALK-4 at approximately 70 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human Activin RIB/ALK‑4 Monoclonal Antibody (Catalog # MAB2221, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

HAF018). A specific band was detected for Activin RIB/ALK‑4 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Activin RIB/ALK‑4 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line mock transfected or transfected with human Activin RIB/ALK-4. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Activin RIB/ALK‑4 Monoclonal Antibody (Catalog # MAB222) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Activin RIB/ALK‑4 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />