AB-108-C, open histogram) followed by Phycoerythrin-conjugated anti-Goat IgG (F0107). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> AB-108-C, open histogram) followed by Phycoerythrin-conjugated anti-Goat IgG (F0107). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="Human peripheral blood monocytes were stained with Goat Anti-Human/Mouse Insulin R/CD220 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1544, filled histogram) or isotype control antibody (AB-108-C, open histogram) followed by Phycoerythrin-conjugated anti-Goat IgG (F0107). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane of hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane of hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB0041, data not shown) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041, data not shown) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="NS0 mouse myeloma cell line transfected with human Insulin R/CD220 (filled histogram), or irrelevant transfectant (open histogram) were stained with Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB15442) or Mouse IgG2B Isotype Control (Catalog # MAB0041, data not shown) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

FAB3832P) and either (A) Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB15442) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> FAB3832P) and either (A) Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB15442) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Insulin R/CD220 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and HDLM‑2 human Hodgkin’s lymphoma cell line (negative control) using Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB1544) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

FAB3832P) and either (A) Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB1544) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> FAB3832P) and either (A) Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB1544) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />