F0101B) and Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002)." class="big_lightbox"> F0101B) and Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002)." alt="Human peripheral blood cells were stained with Human CD96 Monoclonal Antibody (Catalog # MAB6199) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B) and Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002)." class="big_thumb" />

9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96 Fc Chimera (Catalog # 9360-CD) binds with an ED₅₀ of 0.3-1.8 µg/mL." class="big_lightbox"> 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96 Fc Chimera (Catalog # 9360-CD) binds with an ED₅₀ of 0.3-1.8 µg/mL." alt="When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96 Fc Chimera (Catalog # 9360-CD) binds with an ED₅₀ of 0.3-1.8 µg/mL." class="big_thumb" />

HAF016). A specific band was detected for CD96 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for CD96 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human Th2 cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD96 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD96 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

F0113) and Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." class="big_lightbox"> F0113) and Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." alt="Mouse splenocytes were labeled with Mouse CD96 Monoclonal Antibody (Catalog # MAB5690) followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113) and Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." class="big_thumb" />

HAF007). A specific band was detected for CD96v2 at approximately 130 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for CD96v2 at approximately 130 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human Th2 cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CD96v2 Monoclonal Antibody (Catalog # MAB61991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD96v2 at approximately 130 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

10478-CD) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human CD155/PVR Fc Chimera (9174-CD) binds with an ED₅₀ of 6-36 ng/mL." class="big_lightbox"> 10478-CD) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human CD155/PVR Fc Chimera (9174-CD) binds with an ED₅₀ of 6-36 ng/mL." alt="When Recombinant Cynomolgus Monkey CD96 His-tag (10478-CD) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human CD155/PVR Fc Chimera (9174-CD) binds with an ED₅₀ of 6-36 ng/mL." class="big_thumb" />

10478-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-145 kDa." class="big_lightbox"> 10478-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-145 kDa." class="big_thumb" />

9174-CD) with an ED₅₀ of 30.0-300 ng/mL." class="big_lightbox"> 9174-CD) with an ED₅₀ of 30.0-300 ng/mL." alt="Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein (Catalog # AVI9556) binds Recombinant Human CD155/PVR Fc Chimera (9174-CD) with an ED₅₀ of 30.0-300 ng/mL." class="big_thumb" />

6909-CD) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Mouse CD96 Fc Chimera (Catalog # 10421-CD) binds with an ED₅₀ of 4-24 ng/mL." class="big_lightbox"> 6909-CD) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Mouse CD96 Fc Chimera (Catalog # 10421-CD) binds with an ED₅₀ of 4-24 ng/mL." alt="When Recombinant Mouse CD155/PVR (Catalog # 6909-CD) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Mouse CD96 Fc Chimera (Catalog # 10421-CD) binds with an ED₅₀ of 4-24 ng/mL." class="big_thumb" />

9764-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Rat CD96 Fc Chimera (Catalog # 10400-CD) binds with an ED₅₀ of 10-70 ng/mL." class="big_lightbox"> 9764-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Rat CD96 Fc Chimera (Catalog # 10400-CD) binds with an ED₅₀ of 10-70 ng/mL." alt="When Recombinant Rat CD155/PVR (Catalog # 9764-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Rat CD96 Fc Chimera (Catalog # 10400-CD) binds with an ED₅₀ of 10-70 ng/mL." class="big_thumb" />

9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) binds with an ED₅₀ of 30-150 ng/mL." class="big_lightbox"> 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) binds with an ED₅₀ of 30-150 ng/mL." alt="When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) binds with an ED₅₀ of 30-150 ng/mL." class="big_thumb" />